| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Raffinose is a non-digestive prebiotic oligosaccharide that has been reported to significantly increase the number of beneficial gut microbiota, such as Bifidobacterium. The purpose of this study is to evaluate the effects of raffinose dietary supplementation on murine gut microbial ecology using mass spectrometry-based metabolome analysis and analysis of fecal microbial composition using 16S rRNA sequencing. We analyzed male 8-weeks-old BALB/c mice that were fed a diet containing 5% raffinose for a week. Murine fecal metabolites were dramatically changed during after intake of raffinose supplemented diet, especially amino acids were significantly increased, indicating that amino acids might be play a role in prebiotic effects of raffinose degradation and fermentation.
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