Molecular mechanisms of left-right asymmetric differentiation of flounder metamorphosis
| Project/Area Number |
24780178
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Tohoku University |
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Principal Investigator |
YOKOI Hayato 東北大学, (連合)農学研究科(研究院), 助教 (40569729)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ヒラメ / 変態 / 異体類 / 左右非対称化 / サブトラクション |
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| Outline of Final Research Achievements |
Flounder transform their body plan during metamorphosis and turn into asymmetric body form with two eyes on left side and ocular-side pigmentation. Metamorphosis is triggered by thyroid hormone signal, however, little is known about molecular basis of the metamorphic morphogenesis. Here, we compared gene expression profile between ocular and blind side by RNA-seq analysis using next generation sequencer, which identified 32 ocular side dominant genes and 7 blind side dominant genes. These candidates are now under screening by in situ hybridization to confirm left-right biased gene expression. So far, most of them showed biased gene expression as expected, which include known pigmentation genes such as gch2.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] No evidence for AID/MBD4-coupled DNA demethylation in zebrafish embryos.2014
Author(s)
Shimoda, N., Hirose, K., Kaneto, R., Izawa, T., Yokoi, H., Hashimoto, N. and Kikuchi, Y
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Journal Title
PLoS ONE
Volume: 9
Issue: 12
Pages: e114816-e114816
DOI
Related Report
Peer Reviewed / Open Access
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