| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Flounder transform their body plan during metamorphosis and turn into asymmetric body form with two eyes on left side and ocular-side pigmentation. Metamorphosis is triggered by thyroid hormone signal, however, little is known about molecular basis of the metamorphic morphogenesis. Here, we compared gene expression profile between ocular and blind side by RNA-seq analysis using next generation sequencer, which identified 32 ocular side dominant genes and 7 blind side dominant genes. These candidates are now under screening by in situ hybridization to confirm left-right biased gene expression. So far, most of them showed biased gene expression as expected, which include known pigmentation genes such as gch2.
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