Food environment of cultured oyster in Matsushima Bay, Japan

Research Project

Project/Area Number	24780179
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General fisheries
Research Institution	Tohoku University
Principal Investigator	NISHITANI Goh 東北大学, (連合)農学研究科(研究院), 助教 (70450781)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	宮城県松島湾 / 二枚貝幼生 / 種判別 / 餌料環境 / 松島湾 / 判別 / 餌 / 二枚貝 / 幼生 / 分類 / 生態
Outline of Final Research Achievements	Totally 516 natural bivalve larvae collected from Matsushima Bay were isolated and identified by molecular methods. A multiplex PCR assay has been developed that enables simultaneous detection of seven bivalve larvae species on the basis of differences in the lengths of the PCR products. The gut contents were analyzed to clarify the potential food sources of the adult oyster. Using universal primer and gene cloning, sequences of suspended phytoplankton, such as diatom Thalassiosira spp. and dinoflagellate Scrippsiella trochoidea, were mainly detected with other sequences of Bolidophyceae, sea squirts, red algae, ciliates, and copepoda.