Screening of candidate proteins related to susceptibility of fugu to its parasite

• Project/Area Number: 24780182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: The University of Tokyo
• Principal Investigator: TASUMI Satoshi 東京大学, 農学生命科学研究科, 助教 (90359646)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 宿主特異性 / 2次元電気泳動 / MS/MS / トラフグ / 鰓 / 寄生虫 / LC-MS/MS

Research Abstract

A monogenean parasite Heterobothrium okamotoi parasitize gills of Takifugu rubripes, but not those of its relative species T. niphobles, suggesting that the parasite recognize molecules exclusively existing in gills of T. rubripes. To screen such molecules, 2D electrophoresis followed by MALDI-MS/MS analysis, and suppression subtractive hybridization (SSH) techniques were applied. By 2D electrophoresis, at least 34 species specific protein spots were found, among them 18 spots were identified. By SSH, so far 350 candidate sequences have been obtained. In this study, further screening by cell surface display has not yet been completed, however, multiple candidates have already been obtained and a various experimental problems to be solved became apparent. Knowledge obtained by this research mentioned above is helpful for further research on searching host-specificity determining molecules.

Research Products

• [Presentation] トラフグとクサフグの鰓において存在量の異なる膜タンパク質の単離 (2014)
  • Author(s): 田角聡志,山口晶,筒井繁行,中村修,菊池潔,鈴木譲
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-29
• [Presentation] トラフグとクサフグの鰓において存在量の異なる膜タンパク質の単離 (2013)
  • Author(s): 田角聡志・山口晶・筒井繁行・ 中村修・菊池潔・鈴木譲
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京）
• [Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes (2012)
  • Author(s): S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
  • Organizer: 12th Congress of the International Society of Developmental and Comparative Immunology
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-07-10
• [Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes (2012)
  • Author(s): S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
  • Organizer: The 12th Congress of ISDCI
  • Place of Presentation: ヒルトン福岡シーホーク（福岡）
• [Presentation] トラフグとクサフグの鰓において発現量の異なる遺伝子単離の試み (2012)
  • Author(s): 田角聡志・菊池潔・鈴木譲
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校（下関）