Screening of candidate proteins related to susceptibility of fugu to its parasite
Project/Area Number |
24780182
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General fisheries
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Research Institution | The University of Tokyo |
Principal Investigator |
TASUMI Satoshi 東京大学, 農学生命科学研究科, 助教 (90359646)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 宿主特異性 / 2次元電気泳動 / MS/MS / トラフグ / 鰓 / 寄生虫 / LC-MS/MS |
Research Abstract |
A monogenean parasite Heterobothrium okamotoi parasitize gills of Takifugu rubripes, but not those of its relative species T. niphobles, suggesting that the parasite recognize molecules exclusively existing in gills of T. rubripes. To screen such molecules, 2D electrophoresis followed by MALDI-MS/MS analysis, and suppression subtractive hybridization (SSH) techniques were applied. By 2D electrophoresis, at least 34 species specific protein spots were found, among them 18 spots were identified. By SSH, so far 350 candidate sequences have been obtained. In this study, further screening by cell surface display has not yet been completed, however, multiple candidates have already been obtained and a various experimental problems to be solved became apparent. Knowledge obtained by this research mentioned above is helpful for further research on searching host-specificity determining molecules.
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Report
(3 results)
Research Products
(5 results)
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[Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes2012
Author(s)
S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
Organizer
12th Congress of the International Society of Developmental and Comparative Immunology
Place of Presentation
Fukuoka
Year and Date
2012-07-10
Related Report
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[Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes2012
Author(s)
S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
Organizer
The 12th Congress of ISDCI
Place of Presentation
ヒルトン福岡シーホーク(福岡)
Related Report
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