| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Research Abstract |
A monogenean parasite Heterobothrium okamotoi parasitize gills of Takifugu rubripes, but not those of its relative species T. niphobles, suggesting that the parasite recognize molecules exclusively existing in gills of T. rubripes. To screen such molecules, 2D electrophoresis followed by MALDI-MS/MS analysis, and suppression subtractive hybridization (SSH) techniques were applied. By 2D electrophoresis, at least 34 species specific protein spots were found, among them 18 spots were identified. By SSH, so far 350 candidate sequences have been obtained. In this study, further screening by cell surface display has not yet been completed, however, multiple candidates have already been obtained and a various experimental problems to be solved became apparent. Knowledge obtained by this research mentioned above is helpful for further research on searching host-specificity determining molecules.
|
