| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
In this study, transgenic method utilizing cell regeneration of coenocytic green alga Bryopsis plumosa was investigated. The expression vectors where the expression of the reporter genes were controlled by the house keeping gene promoter of B. plumosa were constructed. Although the foreign gene introduction to the algal bodies was attempted by several methods including conventional ones and a new one of which the reporter gene was added into the squeezing medium, the reporter expression was not detected in any of the methods. In addition, the reporter mRNA encoding Renilla luciferase was introduced into the cultivated coenocytic green alga Caulerpa lentillifera by the method where B. plumosa showed the reporter expression in the previous study, however, the luciferase activity was not detected in the cultivated species.
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