Defense mechanism of epidermis in agnathan

• Project/Area Number: 24780193
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Kitasato University
• Principal Investigator: TSUTSUI Shigeyuki 北里大学, 海洋生命科学部, 講師 (20406911)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 無顎類 / 表皮 / 細胞 / レーザーマイクロダイセクション / 次世代シークエンサー / 防御機構

Research Abstract

Among the Agnatha which were the most primitive vertebrate, I developed a method to collect Skein cell group and other cells group from skin frozen section of the sand lamprey (Lethenteron reissneri) using a laser micro disection (LMD) effectively. Furthermore, I extracted mRNA from these two groups. These mRNAs were subjected to next-generation sequencing to try to exhaustive analysis on expressing genes in these cells.