| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Four medaka sialidase genes (neu1, neu3a, neu3b and neu4)has been cloned and characterized. Neu1 and Neu4 were localized at lysosome and their good substrates were oligosaccharides. On the other hand, ganglioside sialidases Neu3a and Neu3b were detected at plasma membrane and cytosol, respectively.
Physiological functions of medaka sialidases were quite different from each other. Neu3a enhanced the differentiation of neurite formation. Neu3b positively regulated myoblast differentiation through the down-regulation of egfr mRNA expression. Neu1 and Neu4 are involved in the catabolism of glycoproteins in lysosome.
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