Candidate gene approach to identify the meadow fescue (Festuca pratensis L.)-derived alleles to improve winter-hardiness of perennial ryegrass (Lolium perenne L.)

• Project/Area Number: 24780260
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Zootechnical science/Grassland science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: TAMURA Ken-ichi 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター酪農研究領域, 主任研究員 (10414749)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: イネ科牧草 / 越冬性 / 遺伝子 / QTL / ペレニアルライグラス / メドウフェスク / イントログレッション / 耐凍性 / DNAマーカー / VRN1 / 分子育種 / ストレス耐性 / 種間雑種 / 移入交雑

Outline of Final Research Achievements

The aim of this study is to identify the genomic regions in meadow fescue (MF, Festuca pratensis L.) associated with QTL to improve winter hardiness in perennial ryegrass (PR, Lolium perenne L.). Perennial ryegrass plants with some introgressions from MF were genetically analyzed using two approach; a candidate gene approach and QTL analysis of back crossed population. Comparison of genotypes (MF homo, PR homo or hetero) of MF-PR introgression plants for five candidate genes revealed that MF-derived allele of Vernalization 1 (VRN1) showed positive effect on the freezing tolerance in the PR background. QTL analysis of winter hardiness in the snow-covered region identified a QTL in the linkage group 7, where the MF allele has positive effect. These results could be applied to the molecular breeding to improve winter hardiness of perennial ryegrass.

Research Products

• [Presentation] フェスク-ロリウム間におけるムギ類耐凍性QTL FR-1, FR-2同祖領域の移入の効果 (2015)
  • Author(s): 田村健一、田瀬和浩、眞田康治、久保田明人、秋山征夫
  • Organizer: 日本草地学会
  • Place of Presentation: 信州大学南箕輪キャンパス
  • Year and Date: 2015-03-26
• [Presentation] メドウフェスク由来候補遺伝子領域のペレニアルライグラスへの移入による耐凍性への影響 (2015)
  • Author(s): 田村健一、田瀬和浩、眞田康治、久保田明人、秋山征夫
  • Organizer: 日本育種学会
  • Place of Presentation: 玉川大学町田キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] メドウフェスクーペレニアルライグラス第７連鎖群の越冬性QTLの同定 (2013)
  • Author(s): 田村健一、田瀬和浩、眞田康治
  • Organizer: 日本育種学会
  • Place of Presentation: 鹿児島大学
• [Presentation] ペレニアルライグラスの越冬性向上に有用なメドウフェスク由来ゲノム領域の探索 (2013)
  • Author(s): 田村健一、田瀬和浩、眞田康治、小松敏憲、久保田明人
  • Organizer: 日本草地学会
  • Place of Presentation: 山形大学
• [Presentation] Molecular breeding for the improvement of winter hardiness in perennial ryegrass (Lolium perenne L.) by introgression of genes from meadow fescue (Festuca pratensis Huds.) (2012)
  • Author(s): 田村健一、田瀬和浩、眞田康治、小松敏憲、久保田明人、米丸淳一
  • Organizer: 7th International Symposium on the Molecular Breeding of Forage and Turf
  • Place of Presentation: アメリカ ソルトレイクシティ
  • Invited
• [Presentation] Comparative transcriptomics between meadow fescue (Festuca pratensis Huds.) and perennial ryegrass (Lolium perenne L.) during cold acclimation (2012)
  • Author(s): 田村健一、田瀬和浩、眞田康治、米丸淳一
  • Organizer: Plant and Microbe Adaptations to Cold, 2012
  • Place of Presentation: 北海道大学
• [Book] Diagnostics in Plant Breeding （分担章名：Insertion-deletion marker targeting intron polymorphisms） (2013)
  • Author(s): 田村健一、米丸淳一、山田敏彦
  • Total Pages: 18
  • Publisher: Springer