• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Candidate gene approach to identify the meadow fescue (Festuca pratensis L.)-derived alleles to improve winter-hardiness of perennial ryegrass (Lolium perenne L.)

Research Project

Project/Area Number 24780260
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Zootechnical science/Grassland science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

TAMURA Ken-ichi  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター酪農研究領域, 主任研究員 (10414749)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsイネ科牧草 / 越冬性 / 遺伝子 / QTL / ペレニアルライグラス / メドウフェスク / イントログレッション / 耐凍性 / DNAマーカー / VRN1 / 分子育種 / ストレス耐性 / 種間雑種 / 移入交雑
Outline of Final Research Achievements

The aim of this study is to identify the genomic regions in meadow fescue (MF, Festuca pratensis L.) associated with QTL to improve winter hardiness in perennial ryegrass (PR, Lolium perenne L.). Perennial ryegrass plants with some introgressions from MF were genetically analyzed using two approach; a candidate gene approach and QTL analysis of back crossed population. Comparison of genotypes (MF homo, PR homo or hetero) of MF-PR introgression plants for five candidate genes revealed that MF-derived allele of Vernalization 1 (VRN1) showed positive effect on the freezing tolerance in the PR background. QTL analysis of winter hardiness in the snow-covered region identified a QTL in the linkage group 7, where the MF allele has positive effect. These results could be applied to the molecular breeding to improve winter hardiness of perennial ryegrass.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2015 2013 2012

All Presentation (6 results) (of which Invited: 1 results) Book (1 results)

  • [Presentation] フェスク-ロリウム間におけるムギ類耐凍性QTL FR-1, FR-2同祖領域の移入の効果2015

    • Author(s)
      田村健一、田瀬和浩、眞田康治、久保田明人、秋山征夫
    • Organizer
      日本草地学会
    • Place of Presentation
      信州大学南箕輪キャンパス
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] メドウフェスク由来候補遺伝子領域のペレニアルライグラスへの移入による耐凍性への影響2015

    • Author(s)
      田村健一、田瀬和浩、眞田康治、久保田明人、秋山征夫
    • Organizer
      日本育種学会
    • Place of Presentation
      玉川大学町田キャンパス
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] メドウフェスクーペレニアルライグラス第7連鎖群の越冬性QTLの同定2013

    • Author(s)
      田村健一、田瀬和浩、眞田康治
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ペレニアルライグラスの越冬性向上に有用なメドウフェスク由来ゲノム領域の探索2013

    • Author(s)
      田村健一、田瀬和浩、眞田康治、小松敏憲、久保田明人
    • Organizer
      日本草地学会
    • Place of Presentation
      山形大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Molecular breeding for the improvement of winter hardiness in perennial ryegrass (Lolium perenne L.) by introgression of genes from meadow fescue (Festuca pratensis Huds.)2012

    • Author(s)
      田村健一、田瀬和浩、眞田康治、小松敏憲、久保田明人、米丸淳一
    • Organizer
      7th International Symposium on the Molecular Breeding of Forage and Turf
    • Place of Presentation
      アメリカ ソルトレイクシティ
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Comparative transcriptomics between meadow fescue (Festuca pratensis Huds.) and perennial ryegrass (Lolium perenne L.) during cold acclimation2012

    • Author(s)
      田村健一、田瀬和浩、眞田康治、米丸淳一
    • Organizer
      Plant and Microbe Adaptations to Cold, 2012
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Research-status Report
  • [Book] Diagnostics in Plant Breeding (分担章名:Insertion-deletion marker targeting intron polymorphisms)2013

    • Author(s)
      田村健一、米丸淳一、山田敏彦
    • Total Pages
      18
    • Publisher
      Springer
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi