Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Outline of Final Research Achievements |
The aim of this study is to identify the genomic regions in meadow fescue (MF, Festuca pratensis L.) associated with QTL to improve winter hardiness in perennial ryegrass (PR, Lolium perenne L.). Perennial ryegrass plants with some introgressions from MF were genetically analyzed using two approach; a candidate gene approach and QTL analysis of back crossed population. Comparison of genotypes (MF homo, PR homo or hetero) of MF-PR introgression plants for five candidate genes revealed that MF-derived allele of Vernalization 1 (VRN1) showed positive effect on the freezing tolerance in the PR background. QTL analysis of winter hardiness in the snow-covered region identified a QTL in the linkage group 7, where the MF allele has positive effect. These results could be applied to the molecular breeding to improve winter hardiness of perennial ryegrass.
|