| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal (BM) embryos were constructed by only oocyte genomes, and therefore, analyses using those embryos enable us to investigate unknown paternally methylated imprinted genes in mouse genome. Comparing transcriptome between wild type (WT) and BM fetuses revealed that Mbnl1 expression in BM those was significantly reduced. We further examined Mbnl1 expression in uniparental fetuses, in which we found that Mbnl1 gene was downregulated in both parthenogenetic and androgenetic fetuses comparing that in WT fetus. These results suggested that Mbnl1 was non-imprinted gene but regulated by imprinted genes during development.
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