The myostatin action on energy metabolism

• Project/Area Number: 24780266
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Kyushu University
• Principal Investigator: TAKAHASHI Hideyuki 九州大学, (連合)農学研究科(研究院), 助教 (60549872)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: myostatin / GLUT4 / なし

Research Abstract

In this study, we aimed to clarify the characteristics of energy metabolism in skeletal muscle of myostatin-deficient Japanese Shorthorn cattle(DM cattle).The expression of GLUT4 mRNA in the skeletal muscle and in myoblasts at 72 h after differentiation in vitro was higher in DM cattle than in NM cattle. Glucose uptake in myoblasts was increased in DM cattle relative to that of NM cattle. Results of the present study suggest that myostatin inhibits the expression of GLUT4 mRNA possibly via MEF2c.

Research Products

• [Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle (2014)
  • Author(s): H. Takahashi, K. Sato, T. Yamaguchi, M. Miyake, H. Watanabe, Y. Nagasawa, E. Kitagawa, S. Terada, M. Urakawa, M. T. Rose, C. D. McMahon, K. Watanabe, S. Ohwada, T. Gotoh, H. Aso
  • Journal Title: Domestic Animal Endocrinology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep (2014)
  • Author(s): Hitoshi Watanabe, Ryo Saito,Tatsuya Nakano, Hideyuki Takahashi, Yu Takahashi, Keisuke Sumiyoshi, Katsuyoshi Sato, Xiangning Chen, Natsumi Okada, Shunsuke Iwasaki, Dian W. Harjanti, Natsumi Sekiguchi, Hiroaki Sano, Haruki Kitazawa, Michael T. Rose, Shyuichi Ohwada, Kouichi Watanabe, Hisashi Aso
  • Journal Title: PLoS ONE Volume: 9 (2)
  • NAID: 120005435471
  • Peer Reviewed
• [Journal Article] 放牧による耕作放棄地解消がイノシシの行動に及ぼす影響 (2014)
  • Author(s): 丸居篤,藤堂乃夫宏,岡安崇史,後藤貴文,衛藤哲次,塩塚雄二,髙橋秀之
  • Journal Title: 日本暖地畜産学会報 Volume: (In press)
  • Peer Reviewed
• [Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double muscled (DM) and normal muscled (NM) Japanese shorthorn cattle. (2014)
  • Author(s): H. Takahashi, K. Sato, T. Yamaguchi, M. Miyake, H. Watanabe, Y. Nagasawa, E. Kitagawa, S. Terada, M. Urakawa, M. T. Rose, C. D. McMahon, K. Watanabe, S. Ohwada, T. Gotoh, H. Aso
  • Journal Title: Domestic Animal Endocrinology Volume: 48 Pages: 62-68
  • DOI: 10.1016/j.domaniend.2014.01.007
  • Peer Reviewed / Open Access
• [Journal Article] Effect of peripheral 5-HT on glucose and lipid metabolism in wether sheep. (2014)
  • Author(s): Watanabe H., Kitazawa H., Watanabe K., Aso H. 他
  • Journal Title: PLoS ONE Volume: 9 Issue: 2 Pages: 1-12
  • DOI: 10.1371/journal.pone.0088058
  • NAID: 120005435471
  • Peer Reviewed
• [Journal Article] Biological rhythms related to metabolism in Japanese Shorthorn cattle under varying environments and management techniques (2013)
  • Author(s): Ogino M, Matsuura A, Yamazaki A, Irimajiri A, Takahashi Hideyuki, Komatsu T, Kushibiki S, Shingu H, Kasuya E, Hasegawa Y, Hodate K
  • Journal Title: Animal Science Journal Volume: 84 Pages: 513-26
  • Peer Reviewed
• [Journal Article] Biological rhythms related to metabolism in Japanese Shorthorn cattle under varying environments and management techniques. (2013)
  • Author(s): Ogino M., Matsuura A., Yamazaki A., Irimajiri A., Takahashi H., Komatsu T., Kushibiki S., Shingu H., Kasuya E., Hasegawa Y., Hodate K.
  • Journal Title: Animal Science Journal Volume: in press
  • Peer Reviewed
• [Journal Article] 放牧牛の飼養管理における血液成分濃度の有用性 (2012)
  • Author(s): 黒瀬陽平,鈴木喜博,髙橋秀之,児島将康,寒川賢治,長谷川喜久,東善行, 小林 茂樹
  • Journal Title: 日本畜産学会報 Volume: 83 Pages: 357-362
  • Peer Reviewed
• [Journal Article] 飼料中タンパ図4.ミオスタチン添加における遺伝子発現ク質含量が血漿脂質成分の日内変動に及ぼす影響 (2012)
  • Author(s): 渡邉一史,高橋辰行,髙橋秀之,佐藤勝祥,加藤和雄,大和田修一,渡邉康一,庄司則章,麻生久
  • Journal Title: 東北畜産学会報 Volume: 62 Pages: 1-5
  • Peer Reviewed
• [Journal Article] AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in Cattle (2012)
  • Author(s): Masato Miyake, Hideyuki Takahashi, Eri Kitagawa, Hitoshi Watanabe, Takahiro Sakurada, Hisashi Aso and Takahiro Yamaguchi
  • Journal Title: CELL & TISSUE RESEARCH Volume: 349 Pages: 612-623
  • Peer Reviewed
• [Presentation] 黒毛和種における初期成長期栄養と筋内細胞外マトリックスの関係に関する研究 (2014)
  • Author(s): 野村碧・齋藤邦彦・佐久間香織・増田恭久・岡田真人・阿部剛・小林栄治・鈴木英敏・金田修一・塩塚雄二・藤村亮佑・衛藤哲次・髙橋秀之・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: つくば市
  • Year and Date: 2014-03-28
• [Presentation] 黒毛和種における初期成長期栄養と筋内細胞外マトリックスの関係に関する研究 (2014)
  • Author(s): 野村碧・齋藤邦彦・佐久間香織・増田恭久・岡田真人・阿部剛・小林栄治・鈴木英敏・金田修一・塩塚雄二・藤村亮佑・衛藤哲次・髙橋秀之・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: つくば国際会議場
• [Presentation] 黒毛和種における初期成長期栄養と筋内細胞外マトリックスの関係に関する研究 (2013)
  • Author(s): 野村碧・齋藤邦彦・佐久間香織・増田恭久・岡田真人・阿部剛・小林栄治・鈴木英敏・金田修一・塩塚雄二・藤村亮佑・衛藤哲次・髙橋秀之・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島市
  • Year and Date: 2013-03-30
• [Presentation] 哺乳期の給与飼料の違いが黒毛和種仔牛の成長および胸最長筋における栄養素代謝に及ぼす影響 (2013)
  • Author(s): 髙橋秀之・木村優里・齋藤昭・猪内勝利・秋月亮介・野村碧・藤村亮祐・塩塚雄二・衛藤哲次・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島市
  • Year and Date: 2013-03-30
• [Presentation] 哺乳期の給与飼料の違いが黒毛和種仔牛の胸最長筋における脂肪形成にかかわる遺伝子群の発現への影響 (2013)
  • Author(s): 木村優里・髙橋秀之・齋藤昭・猪内勝利・秋月亮介・野村碧・藤村亮祐・塩塚雄二・衛藤哲次・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島市
  • Year and Date: 2013-03-30
• [Presentation] 黒毛和種仔牛における哺育形態の違いが肝の免疫および成長系因子の発現に与える影響 (2013)
  • Author(s): 秋月亮介・木村優里・齋藤昭・猪内勝利・野村碧・藤村亮祐・塩塚雄二・衛藤哲次・髙橋秀之・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島市
  • Year and Date: 2013-03-30
• [Presentation] 牛のバイタルデータセンシングを実現するインプラント無線機の研究開発 (2013)
  • Author(s): 西土井健・横尾郁・杉山準・石川頌平・塩塚雄二・衛藤哲次・髙橋秀之・後藤貴文
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島市
  • Year and Date: 2013-03-30
• [Presentation] Metabolic imprinting effect during Early Growth on Extra Cellular Matrix Construction in Japanese Black (2013)
  • Author(s): A Nomura, R Fujimura, S Khounsakunalath, K Saito, K Sakuma, T Abe, S Kaneda, T Etoh, Y Shiotsuka, H Takahashi, T Gotoh
  • Organizer: The 8th World Congress on Developmental Origins of Health and Disease (DOHaD)
  • Place of Presentation: SUNTEC, Singapore
• [Presentation] Metabolic Imprinting Effect in Beef Production : Influence of Nutrition Manipulation during an Early Growth Stage on PPARγ2 and PMRT5 Expressions in the Longissimus Muscle in Wagyu (Japanese Black) (2013)
  • Author(s): Sithyphone K, R Fujimura, K Etoh, K Saito, K Sakuma, K Kaneda, T Abe, T Etoh, Y Shiotsuka, H Hasebe, H Hasebe, S Maak, A Elke, H Takahashi, A Saito, T, Gotoh
  • Organizer: The proceedings of the 8th Developmental Origins of Health and Disease (DOHaD)
  • Place of Presentation: SUNTEC Singapore
• [Presentation] Metabolic imprinting effect during Early Growth on Extra Cellular Matrix Construction in Wagyu (Japanese Black Steers) (2013)
  • Author(s): A Nomura, R Fujimura, A Saito, S Khounsakunalath, K Saito, K Sakuma, T Abe, H Hasebe, S Kaneda, T Etoh, Y Shiotsuka, S Maak, E Albrecht, H Takahashi, T Gotoh
  • Organizer: Joint Annual Meeting 2013 in Indiana
  • Place of Presentation: USA
• [Presentation] The effect of nutrients of milk replacer on gene expressions related to intramuscular adipogenesis in longissimus muscle of Japanese Black calves (2013)
  • Author(s): Y. Kimura, H. Takahashi, A. Saito, K. Inouti, A. Nomura, Y. Shiotsuka, T. Etoh and T. Gotoh
  • Organizer: The AFELiSA 2013
  • Place of Presentation: Miyazaki, Japan
• [Presentation] Comparison of intramuscular fat contents between muscles of Japanese Black heifers (Wagyu) fed with different nutrients (2013)
  • Author(s): Sithyphone, K., T, Fumita., A, Matsubara., Y, Shiotsuka., T, Etoh., H, Takahashi., T, Gotoh
  • Organizer: The proceedings of the 10th International Joint Symposium among Japan, Korea and China, Topic : International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA)
  • Place of Presentation: Miyazaki, Japan
• [Presentation] Preliminary Report : The Pattern of Intramuscular Fat Accumulation of Several Kinds of Muscles with Growth in Wagyu (2013)
  • Author(s): Atsuko Matsubara, Khounsaknalath Sithyphone, Kumihito Sakashita, Kenji Kawabata, Hideyuki Takahashi, Yuji Shiotsuka, Tetsuji Etoh and Takahumi Gotoh
  • Organizer: The proceedings of the 10th International Joint Symposium among Japan, Korea and China, Topic : International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA)
  • Place of Presentation: Sun Hotel, Miyazaki, Japan
• [Presentation] 黒毛和牛における哺乳期の給与飼料の差異が離乳直前の筋内脂肪形成関連遺伝子群の発現に与える影響 (2013)
  • Author(s): 木村優里・髙橋秀之・齋藤昭・猪内勝利・野村碧・藤野亮一・塩塚雄二・衛藤哲次・後藤貴文
  • Organizer: 第6回日本暖地畜産学会
  • Place of Presentation: 鹿児島市
• [Presentation] 黒毛和牛における哺乳期の給与飼料の差異が離乳直前の筋内脂肪形成関連遺伝子群の発現に与える影響 (2013)
  • Author(s): ② 木村優里・髙橋秀之・齋藤昭・猪内勝利・野村碧・藤野亮一・塩塚雄二・衛藤哲次・後藤貴文
  • Organizer: 第6回日本暖地畜産学会
  • Place of Presentation: 鹿児島大学
• [Presentation] 黒毛和種における初期成長期栄養と筋内細胞外マトリックスの関係に関する研究 (2013)
  • Author(s): 野村碧、齋藤邦彦、佐久間香織、増田恭久、岡田真人、阿部剛、小林栄治、鈴木英敏、金田修一、塩塚雄二、藤村亮祐、衛藤哲次、高橋秀之、後藤貴文
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] 哺乳期の給与飼料の違いが黒毛和種仔牛の成長および胸最長筋における栄養素代謝に及ぼす影響 (2013)
  • Author(s): 高橋秀之、木村優里、齋藤昭、猪内勝利、秋月亮介、野村碧、藤村亮祐、塩塚雄二、衛藤哲次、後藤貴文
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] 哺乳期の給与飼料の違いが黒毛和種仔牛の胸最長筋における脂肪形成に関わる遺伝子群のへ発現への影響 (2013)
  • Author(s): 木村優里、高橋秀之、齋藤昭、猪内勝利、秋月亮介、野村碧、藤村亮祐、塩塚雄二、衛藤哲次、後藤貴文
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] 草原短角牛への稲ホールクロップサイレージ給与による血漿成分の変動 (2013)
  • Author(s): 佐藤勝祥、浦川めぐみ、高橋秀之、佐藤光美、藤本長之、佐藤富雄、渡邊康一、麻生久
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] 黒毛和種仔牛における哺乳形態の違いが肝の免疫および成長系因子の発現に与える影響 (2013)
  • Author(s): 秋月亮介、木村優里、齋藤昭、猪内勝利、野村碧、藤村亮祐、塩塚雄二、衛藤哲次、高橋秀之、後藤貴文
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] 牛のバイタルデータセンシングを実現するインプラント無線機の研究開発 (2013)
  • Author(s): 西土井健、横尾郁、杉山準、石川頌平、塩塚雄二、衛藤哲次、高橋秀之、後藤貴文
  • Organizer: 日本畜産学会
  • Place of Presentation: 広島大学
• [Presentation] Relationship between early nutrition and extra cellular matrix construction in Japanese Black cattle (2012)
  • Author(s): Aoi Nomura, Kunihiko Saitou, Kaori Sakuma, Tsuyoshi Abe, Eiji Kobayashi, Hidetoshi Suzuki, Syuichi Kaneda, Akira Saito, Yuji Shiotsuka, Ryosuke Fujimura, Tetsuji Etoh, Hideyuki Takahashi and Takafumi Gotoh
  • Organizer: The Proceedings of International Symposium on Agriculture, Food, Environmental and Life Science in Asia 2012 in Deajeon
  • Place of Presentation: Korea
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/lab/kuju_hp/