| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
In the mouse, L-amino acid oxidase is known as anti-bacterial factor by producing H2O2 in mammary glands. In the dairy cattle, mastitis is the most frequent disease and the expression level of LAO mRNA is considerably lower than that in mice. In this studies, we generated recombinant LAO protein encoding bovine LAO using silkworms system and confirmed the ability for production of H2O2 in vitro. We also generated bovine LAO expressing Tg mice. The generated Tg mice will be useful animal model to investigate whether bovine LAO reveals anti-bacterial effect in vivo and to indicate LAO gene is strong candidate for the production of mastitis-resistant cattle.
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