| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Research Abstract |
The creation of an efficient technique for producing transgenic chicks may lead to industrial applications in agriculture and biopharmacy; moreover, it will advance our understanding of avian biology itself. The objective of this study was to investigate the method for producing transgenic avian species by injected piggyBac transposon constructs into blastoderm after laying the egg. Consequently, when 2.5ng/ul piggyBac transposase plasmid was injected into the egg blastoderm, it could be introduced for gene transferred transgenic chicks.
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