| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
In this study, we focused on progesterone (P4) deactivation enzyme, AKR1C1, which expression had been firstly found in our laboratory during follicular development in pig, and investigated for revealing follicular selection system of dominant follicle by measuring expression pattern of the enzyme and the other gene for estrogen (E2) and P4 synthesis (E2; Cyp19a1, P4; Star, Cyp11a1, Hsd3b1) and concentration of E2 and P4 in follicular fluid. Furthermore, we exploited the novel in vitro development (IVG) and in vitro maturation (IVM) system based on above data.
As the results, we showed that expression pattern of Akr1c1 mRNA and E2/P4 synthesis gene were different between dominant follicle and regression follicle, resulting in differing E2 and P4 concentration in these follicular fluid. In addition, based on E2 and P4 level in dominant follicle, we invented novel IVG-IVM system that can produce blastocyst with high efficiency derived from regression follicle.
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