Regulation of species--dependent calcium oscillations during fertilization in mammals

• Project/Area Number: 24780272
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Azabu University
• Principal Investigator: ITO JUNYA 麻布大学, 獣医学部, 准教授 (30454143)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 受精

Research Abstract

We succeeded in molecular cloning of horse PLCz. We also showed that horse PLCz has higher activity compared to those in other mammalian species studied to date. As for the regulation of IP3R in pig oocytes, inhibition of polo-like kinase 1(Plk1) dramatically decreased the expression level of IP3R, suggests that Plk1 is involved in the regulation of IP3R expression at least in pig oocytes.

Research Products

• [Journal Article] The effect of a novel cryoprotective agent, carboxylated ε-poly-l-lysine, on the developmental ability of re-vitrified mouse embryos at the pronuclear stage (2014)
  • Author(s): Shibao Y, Fujiwara K, Kawasaki Y, Matsumura K, Hyon SH, Ito J, Kashiwazaki N.
  • Journal Title: Cryobiology Volume: 68 Issue: 2 Pages: 200-204
  • DOI: 10.1016/j.cryobiol.2014.01.008
  • Peer Reviewed
• [Journal Article] Molecular characteristics of horse phospholipase C zeta (PLCz) (2013)
  • Author(s): Kana Sato, Takuya Wakai, Yasunari Seita, Akiko Takizawa, Rafael A. Fissore, Junya Ito, Naomi Kashiwazaki
  • Journal Title: Animal Science Journal Volume: 84 Issue: 4 Pages: 359-368
  • DOI: 10.1111/asj.12044
  • Peer Reviewed
• [Journal Article] 〓neration of Live Offspring from Vitrified Mouse Oocytes of C57BL/6J Strain (2013)
  • Author(s): Natsuki Kohaya, Katsuyoshi Fuiiwara, Junya Ito, Naomi Kashiwazaki
  • Journal Title: PLOS ONE Volume: 8 Issue: 3 Pages: e58063-e58063
  • DOI: 10.1371/journal.pone.0058063
  • Peer Reviewed
• [Journal Article] Vitrification procedure decreases inositol 1,4,5-trisphophate receptor expression, resulting in low fertility of pig oocytes (2013)
  • Author(s): Masahiko Hirose, Maki Kamoshita, Katsuyoshi Fujiwara, Tsubasa Kato, Ayaka Nakamura, Richard Wojcikiewicz, Jan B. Parys, Junya Ito, Naomi Kashiwazaki
  • Journal Title: Animal Science Journal Volume: 84 Issue: 10 Pages: 693-701
  • DOI: 10.1111/asj.12061
  • Peer Reviewed
• [Journal Article] Efficient Production of Live Offspring from Mouse Oocytes Vitrified with a Novel Cryoprotective Agent, Carboxylated ε -poly-L-lysine (2013)
  • Author(s): Watanabe H, Kohaya N, Kamoshita M, Fujiwara K, Matsumura K, Hyon SH, Ito J, Kashiwazaki N.
  • Journal Title: PLoS One Volume: 23 Issue: 12 Pages: e83613-e83613
  • DOI: 10.1371/journal.pone.0083613
  • Peer Reviewed
• [Journal Article] Molecular mechanism of fertilization in the pig (2012)
  • Author(s): Junya Ito, Naomi Kashiwazaki
  • Journal Title: Animal Science Journal Volume: 83 Issue: 10 Pages: 669-682
  • DOI: 10.1111/j.1740-0929.2012.01044.x
  • Peer Reviewed
• [Journal Article] The effect of M-phase stage-dependent kinase inhibitors on inositol 1,4,5-trisphosphate receptor 1 (IP_3R1) expression and localization in pig oocytes
  • Author(s): Anucha Sathanawongs, Katsuyoshi Fujiwara, Tsubasa Kato, Masahiko Hirose, Maki Kamoshita, Richard J.H. Wojcikiewicz, Jan B. Parys, Junya Ito, Naomi Kashiwazaki
  • Journal Title: Animal Science Journal Volume: (in press)
  • Peer Reviewed
• [Presentation] Characteristics of transgenic rats expressing horse PLCz (2013)
  • Author(s): Junya Ito, Yuki Yamashita, Koji Furukawa, Ayaka Nakamura, Kana Sato, Akiko Takizawa, Hoi Chang Lee, Rafael A. Fissore, Naomi Kashiwazaki
  • Organizer: Gordon Research Conference
  • Place of Presentation: アメリカ合衆国
• [Presentation] Activation of round spermatid-injected oocytes using phospholipase C zeta in pigs (2013)
  • Author(s): Junya Ito, Emi Yuhara, Ayaka Nakamura, Naomi Kashiwazaki
  • Organizer: Annual Meeting of International Embryo Tranafer Society
  • Place of Presentation: ドイツ
• [Presentation] Activation of round spermatid-injected oocytes using phospholipase C zeta in pigs (2013)
  • Author(s): Junya Ito, Emi Yuhara, Ayaka Nakamura, Naomi Kashiwazaki
  • Organizer: Annual Meeting of International Embryo Transfer Society
  • Place of Presentation: ドイツ
• [Presentation] Improvement of in vitro and in vivo development of vitrified mammalian oocytes and embryos (2012)
  • Author(s): Junya Ito
  • Organizer: Annual Meeting of Asia Reproductive Biology Society
  • Place of Presentation: フィリピン
• [Presentation] Characteristics of transgenic rats expressing horse PLCz
  • Author(s): Junya Ito, Yuki Yamashita, Koji Furukawa, Ayaka Nakamura, Kana Sato, Akiko Takizawa, Hoi Chang Lee, Rafael A. Fissore, Naomi Kashiwazaki
  • Organizer: Gordon Research Conference（GRC 2013）
  • Place of Presentation: Holderness School, Holderness, NH, United States
• [Book] The Ca^<2+> signal at fertilization in mammals: PLC, sterility, and species-specific differences in activity. Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons Chapter 10: (2012)
  • Author(s): Junya Ito and Rafael A. Fissore
• [Book] Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons (2012)
  • Author(s): Junya Ito and Rafel A. Fissore
  • Total Pages: 239
  • Publisher: Adthree Publishing