Analysis of epigenetic reprogramming during germ cell development in cloned cattle

• Project/Area Number: 24780273
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: KANEDA Masahiro 東京農工大学, (連合)農学研究科(研究院), 助教 (80469840)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: クローン / エピジェネティクス / DNAメチル化 / 精子 / 卵子 / 生殖細胞 / インプリント遺伝子 / クローン牛

Research Abstract

There are lots of reports showing developmental defects in somatic cell nuclear transferred animals (cloned animals). However, these abnormalities are not inherited to the next generation via normal fertilization process. It is assumed that epigenetic errors occurred in cloned animals were reset during germ cell development. In this project, we analyzed DNA methylation levels in sperm and oocytes derived from cloned cattle and non-cloned control ones. All genes and regions analyzed were not different between cloned and non-clones.

Research Products

• [Journal Article] Production of Fertile Offspring from Oocytes Grown In Vitro by Nuclear Transfer in Cattle. (2013)
  • Author(s): Hirao Y, Naruse K, Kaneda M, Somfai T, Iga K, Shimizu M, Akagi S, Cao F, Kono T, Nagai T, Takenouchi N.
  • Journal Title: Biology of Reproduction Volume: 89 Issue: 3 Pages: 47-67
  • DOI: 10.1095/biolreprod.113.109439
  • Peer Reviewed / Open Access
• [Journal Article] Naloxone increases maturation rate and ratio of inner cell mass to total cells in blastocysts in pigs. (2013)
  • Author(s): Dang-Nguyen TQ, Viet Linh N, Minoia R, Kaneda M, Somfai T, Haraguchi S, Akagi S, Kikuchi K, Nakai M, Tajima A, Nagai T.
  • Journal Title: Animal Science Journal Volume: 84 Issue: 12 Pages: 765-773
  • DOI: 10.1111/asj.12071
  • Peer Reviewed
• [Journal Article] Downregulation of histone methyltransferase genes SUV39H1 and SUV39H2 increases telomere length in embryonic stem-like cells and embryonic fibroblasts in pigs (2013)
  • Author(s): Dang-Nguyen TQ, Haraguchi S, Furusawa T, Somfai T, Kaneda M, Watanabe S, Akagi S, Kikuchi K, Tajima A, Nagai T
  • Journal Title: J Reprod Dev Volume: 59(1) Pages: 27-32
  • NAID: 10031156808
  • URL: https://www.jstage.jst.go.jp/article/jrd/59/1/59_2012-118/_article
  • Peer Reviewed
• [Journal Article] Supplementation of culture medium with L-carnitine improves development and cryotolerance of bovine embryos produced in vitro (2013)
  • Author(s): Takahashi T, Inaba Y, Somfai T, Kaneda M, Geshi M, Nagai T, Manabe N
  • Journal Title: Reprod Fertil Dev Volume: 25(4) Issue: 4 Pages: 589-99
  • DOI: 10.1071/rd11262
  • Peer Reviewed
• [Journal Article] Characteristics of bovine inner cell mass-derived cell lines and their fate in chimeric conceptuses (2013)
  • Author(s): Furusawa T, Ohkoshi K, Kimura K, Matsuyama S, Akagi S, Kaneda M, Ikeda M, Hosoe M, Kizaki K, Tokunaga T
  • Journal Title: Biology of Reproduction Volume: 89（2）：28 Issue: 2 Pages: 1-12
  • DOI: 10.1095/biolreprod.112.106641
  • Peer Reviewed
• [Journal Article] Downregulation of Histone Methyltransferase Genes <i>SUV39H1</i> and <i>SUV39H2</i> Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs (2013)
  • Author(s): Dang-Nguyen TQ, Haraguchi S, Furusawa T, Somfai T, Kaneda M, Watanabe S, Akagi S, Kikuchi K, Tajima A, Nagai T.
  • Journal Title: Journal of Reproduction and Development Volume: 59 Issue: 1 Pages: 27-32
  • DOI: 10.1262/jrd.2012-118
  • NAID: 10031156808
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Age-related changes in gene expression of the growth hormone secretagogue and growth hormone-releasing hormone receptors in Holstein-Friesian cattle (2012)
  • Author(s): Komatsu M, Kojima M, Okamura H, Nishio M, Kaneda M, Kojima T, Takeda H, Malau-Aduli AE, Takahashi H
  • Journal Title: Domest Anim Endocrinol Volume: 42(2) Issue: 2 Pages: 83-93
  • DOI: 10.1016/j.domaniend.2011.09.006
  • Peer Reviewed
• [Journal Article] Telomere elongation during morula-to-blastocyst transition in cloned porcine embryos (2012)
  • Author(s): Dang-Nguyen TQ, Haraguchi S, Akagi S, Somfai T, Kaneda M, Watanabe S, Kikuchi K, Tajima A, Nagai T
  • Journal Title: Cell Reprogram Volume: 14(6) Issue: 6 Pages: 514-519
  • DOI: 10.1089/cell.2012.0045
  • Peer Reviewed
• [Journal Article] Influence of Intergeneric/interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells (2012)
  • Author(s): Takeda K, Srirattana K, Matsukawa K, Akagi S, Kaneda M, Tasai M, Nirasawa K, Pinkert CA, Parnpai R, Nagai T
  • Journal Title: J Reprod Dev Volume: 58 Pages: 323-329
  • NAID: 10030821280
  • URL: https://www.jstage.jst.go.jp/article/jrd/58/3/58_2011-013/_article
  • Peer Reviewed
• [Journal Article] Follicular Growth-Stimulated Cows Provide Favorable Oocytes for Producing Cloned Embryos (2012)
  • Author(s): Sugimura S, Kobayashi S, Hashiyada Y, Ohtake M, Kaneda M, Yamanouchi T, Matsuda H, Aikawa Y, Watanabe S, Nagai T, Kobayashi E, Konishi K, Imai K
  • Journal Title: Cell Reprogram Volume: 14 Issue: 1 Pages: 29-37
  • DOI: 10.1089/cell.2011.0060
  • Peer Reviewed
• [Journal Article] Effects of the timing of cumulus cell removal from bovine oocytes on enucleation rate and subsequent development after somatic cell nuclear transfer (2012)
  • Author(s): Akagi S, Shiraishi T, Somfai T, Kaneda M, Haraguchi S, Watanabe S
  • Journal Title: J Reprod Dev Volume: 58(5) Pages: 615-9
  • NAID: 10031123308
  • URL: https://www.jstage.jst.go.jp/article/jrd/58/5/58_2012-042/_article
  • Peer Reviewed
• [Journal Article] The Effect of Ovary Storage and In Vitro Maturation on mRNA Levels in Bovine Oocytes; A Possible Impact of Maternal ATP1A1 on Blastocyst Development in Slaughterhouse-derived Oocytes (2012)
  • Author(s): Somfai T, Imai K, Kaneda M, Akagi S, Watanabe S, Haraguchi S, Mizutani E, Dang-Nguyen TQ, Inaba Y, Geshi M, Nagai T
  • Journal Title: J Reprod Dev Volume: 57 Pages: 723-730
  • NAID: 130001170173
  • URL: https://www.jstage.jst.go.jp/article/jrd/57/6/57_11-020H/_article
  • Peer Reviewed
• [Journal Article] Telomere elongation during morula-to-blastocyst transition in cloned porcine embryos. (2012)
  • Author(s): Dang-Nguyen TQ, Haraguchi S, Akagi S, Somfai T, Kaneda M, Watanabe S, Kikuchi K, Tajima A, Nagai T.
  • Journal Title: Cell Reprogram. Volume: 14 Pages: 514-519
  • Peer Reviewed
• [Journal Article] Effects of the timing of cumulus cell removal from bovine oocytes on enucleation rate and subsequent development after somatic cell nuclear transfer. (2012)
  • Author(s): Akagi S, Shiraishi T, Somfai T, Kaneda M, Haraguchi S, Watanabe S.
  • Journal Title: J Reprod Dev. Volume: 58 Pages: 615-619
  • NAID: 10031123308
  • Peer Reviewed
• [Journal Article] Influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells. (2012)
  • Author(s): Takeda K, Srirattana K, Matsukawa K, Akagi S, Kaneda M, Tasai M, Nirasawa K, Pinkert CA, Parnpai R, Nagai T.
  • Journal Title: J Reprod Dev. Volume: 58 Pages: 323-329
  • NAID: 10030821280
  • Peer Reviewed
• [Presentation] 同一ドナー細胞由来の体細胞クローン牛を用いたエピジェネティクス解析 (2013)
  • Author(s): 金田正弘、伊藤強、渡辺伸也、赤木悟史、平子誠、下司雅也、永井卓
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2013-09-20
• [Presentation] EPIGENETICS OF CLONED CATTLE, THEIR GERM CELLS AND OFFSPRING (2013)
  • Author(s): Masahiro Kaneda
  • Organizer: 2013Bimodulation Symposum "From Germplasm to Cloning"
  • Place of Presentation: 韓国・ソウル国立大学校
  • Year and Date: 2013-06-05
• [Presentation] 同一ドナー細胞由来の体細胞クローン牛におけるミトコンドリアDNAコピー数・関連遺伝子のメチル化解析 (2013)
  • Author(s): 金田正弘、伊藤強、渡辺伸也、赤木悟、平子誠、武田久美子、下司雅也、永井卓
  • Organizer: 第7回エピジェネティクス研究会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2013-05-30
• [Presentation] Epigenetic modifications of cloned buffalo embryos (2013)
  • Author(s): Masahiro KANEDA, Kumiko TAKEDA, Takashi NAGAI, Kanokwan SRIRATTANA and Rangsun PARNPAI
  • Organizer: 第10回国際バッファロー会議
  • Place of Presentation: タイ国プーケット市(プレワークショップ)
  • Year and Date: 2013-05-05
• [Presentation] Epigenetic modifications of cloned buffalo embryos (2013)
  • Author(s): Masahiro KANEDA, Kumiko TAKEDA, Takashi NAGAI, Kanokwan SRIRATTANA and Rangsun PARNPAI
  • Organizer: 第10回国際バッファロー会議プレワークショップ
  • Place of Presentation: タイ国プーケット市
  • Invited
• [Presentation] 同一ドナー細胞由来の体細胞クローン牛におけるミトコンドリアDNAコピー数・関連遺伝子のメチル化解析 (2013)
  • Author(s): 金田正弘、伊藤強、渡辺伸也、赤木悟、平子誠、武田久美子、下司雅也、永井卓
  • Organizer: 第7回エピジェネティクス研究会
  • Place of Presentation: 奈良県奈良市
• [Presentation] EPIGENETICS OF CLONED CATTLE, THEIR GERM CELLS AND OFFSPRING (2013)
  • Author(s): Masahiro Kaneda
  • Organizer: 2013Bimodulation Symposum "From Germplasm to Cloning"
  • Place of Presentation: 韓国ソウル市
  • Invited
• [Presentation] 同一ドナー細胞由来の体細胞クローン牛を用いたエピジェネティクス解析 (2013)
  • Author(s): 金田正弘、伊藤強、渡辺伸也、赤木悟史、平子誠、下司雅也、永井卓
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] ウシ精子核DNAのメチル化およびミトコンドリアDNAコピー数と受胎性との関連 (2012)
  • Author(s): 金田正弘,渡辺伸也,平尾雄二,赤木悟史,原口清輝,ソムファイタマス,武田久美子,内山京子
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学
  • Year and Date: 2012-09-06
• [Presentation] ウシ精子におけるDNAメチル化レベルおよびミトコンドリアコピー数と受胎性との関連性の解 (2012)
  • Author(s): 金田正弘、渡辺伸也、赤木悟史、原口清輝、ソムファイタマス、武田久美子、内山京子
  • Organizer: 第6回エピジェネティクス研究会
  • Place of Presentation: 学術総合センター
  • Year and Date: 2012-05-14
• [Presentation] ウシ精子におけるDNAメチル化レベルおよびミトコンドリアコピー数と受胎性との関連性の解析 (2012)
  • Author(s): 金田正弘、渡辺伸也、赤木悟史、原口清輝、ソムファイタマス、武田久美子、内山京子
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター（東京都）
• [Presentation] ウシ精子核DNAのメチル化およびミトコンドリアDNAコピー数と受胎性との関連 (2012)
  • Author(s): 金田正弘，渡辺伸也，平尾雄二，赤木悟史，原口清輝，ソムファイタマス，武田久美子，内山京子
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学（茨城県）
• [Remarks] 哺乳類のエピジェネティクス解析
  • URL: http://www.rd.tuat.ac.jp/activities/factors/search/20140401_43.html