Difference in global gene expression profiles in the bovine corpus luteum of the estrous cycle and pregnancy
| Project/Area Number |
24780276
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied animal science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
SAKUMOTO RYOSUKE 独立行政法人農業生物資源研究所, 動物生産生理機能研究ユニット, 主任研究員 (20343999)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ウシ / 妊娠 / 黄体 / 受胎率 / 繁殖 / マイクロアレイ / ケモカイン / コラーゲン / 生殖周期 / 子宮 |
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| Research Abstract |
Functional differences between the corpus luteum (CL) of pregnancy and CL of the cycle in cows were examined. Microarray analysis using a custom-made, 15 K bovine oligo DNA microarray detected 138, 265 and 455 differentially expressed genes in the bovine CL of day 20 to 25, 40 to 45, and 150 to 160 of pregnancy compared with day 10 to 12 (mid-luteal) of the estrous cycle (>2-fold change relative to mid-luteal stage). These up- and down-regulated genes in the CL of pregnancy involved chemokines, growth factor-related proteins, cluster of differentiation molecules, or collagens. The expression of eotaxin, lymphotactin and ENA-78, and their receptors were observed in the CL of pregnancy. Moreover, mRNAs of collagen and fibronectin expression in the pregnant CL were significantly lower than that in the CL of the estrous cycle. Thus, the different gene expression profiles may contribute to functional differences between the CL of pregnancy and the CL of the estrous cycle in cows.
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Report
(3 results)
Research Products
(6 results)
