| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Live-cell imaging analysis revealed that most of mouse cloned embryos and ICSI embryos with freeze-dried spermatozoa occurred abnormal chromosome segregation (ACS). We found that cloned embryos formed micro pronuclei proceeding with time after donor cell injection and most of such embryos occurred ACS in subsequent development. To find cell types with high cloning efficiency, we also sought less amount of a repressive histone mark, dimethylated histone H3 lysine 9 (H3K9m2), and identified CA1 pyramidal cells in the hippocampus and Purkinje cells in the cerebellum as candidates. Using CA1 cells, cloned offspring were obtained at high rates, reaching 10%. This is for the first time demonstrated that adult neurons could be cloned by nuclear transfer. Furthermore, our data may imply that reduced H3K9me2 and increased histone acetylation would act synergistically to improve development of cloned embryos.
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