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Searching for prerequisites for full term development of cloned embryos based on the live cell imaging analysis

Research Project

Project/Area Number 24780277
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied animal science
Research InstitutionUniversity of Yamanashi

Principal Investigator

MIZUTANI Eiji  山梨大学, 総合研究部, 助教 (80443034)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsクローン / ライブセルイメージング / 初期胚発生 / 核移植 / 成体脳神経細胞 / イメージング / 胚発生
Outline of Final Research Achievements

Live-cell imaging analysis revealed that most of mouse cloned embryos and ICSI embryos with freeze-dried spermatozoa occurred abnormal chromosome segregation (ACS). We found that cloned embryos formed micro pronuclei proceeding with time after donor cell injection and most of such embryos occurred ACS in subsequent development. To find cell types with high cloning efficiency, we also sought less amount of a repressive histone mark, dimethylated histone H3 lysine 9 (H3K9m2), and identified CA1 pyramidal cells in the hippocampus and Purkinje cells in the cerebellum as candidates. Using CA1 cells, cloned offspring were obtained at high rates, reaching 10%. This is for the first time demonstrated that adult neurons could be cloned by nuclear transfer. Furthermore, our data may imply that reduced H3K9me2 and increased histone acetylation would act synergistically to improve development of cloned embryos.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Remarks (3 results)

  • [Journal Article] Generation of cloned mice from adult neurons by direct nuclear transfer.2015

    • Author(s)
      Mizutani E, Oikawa M, Kassai H, Inoue K, Shiura H, Hirasawa R, Kamimura S, Matoba S, Ogonuki N, Nagatomo H, Abe K, Wakayama T, Aiba A, Ogura A.
    • Journal Title

      Biology of Reproduction

      Volume: 92(3) 81 Pages: 1-11

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] RNA sequencing-based identification of aberrant imprinting in cloned mice.2014

    • Author(s)
      Okae H, Matoba S, Nagashima T, Mizutani E, Inoue K, Ogonuki N, Chiba H, Funayama R, Tanaka S, Yaegashi N, Nakayama K, Sasaki H, Ogura A, Arima T.
    • Journal Title

      Hum Mol Genet.

      Volume: 23 Issue: 4 Pages: 992-1001

    • DOI

      10.1093/hmg/ddt495

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Successful Serial Recloning in the Mouse over Multiple Generations.2013

    • Author(s)
      Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T.
    • Journal Title

      Cell Stem Cell

      Volume: 12 Issue: 3 Pages: 293-297

    • DOI

      10.1016/j.stem.2013.01.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウスクローン胚のライブセルイメージングと単一胚移植による解析2013

    • Author(s)
      水谷英二、山縣一夫、若山照彦
    • Organizer
      第54回日本卵子学会
    • Place of Presentation
      学術総合センター (東京都千代田区)
    • Related Report
      2013 Research-status Report
  • [Presentation] Live-cell imaging analysis of mouse oocytes injected with freeze-dried spermatozoa.2013

    • Author(s)
      水谷英二、若山清香、岸田佳奈、若山照彦
    • Organizer
      第10回Annual Conference of Asian Reproductive Biotechnology Society
    • Place of Presentation
      ベトナム ムイネ シーリンクビーチリゾート
    • Related Report
      2013 Research-status Report
  • [Presentation] 凍結乾燥精子で受精したマウス初期胚のライブセルイメージング解析2013

    • Author(s)
      水谷英二、若山清香、岸田佳奈、若山照彦
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京農工大学農学部府中キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] マウスクローン胚のライブセルイメージングと単一胚移植による解析2013

    • Author(s)
      水谷英二、山縣一夫、若山照彦
    • Organizer
      題54回 日本卵子学会
    • Place of Presentation
      東京都 学術総合センター
    • Related Report
      2012 Research-status Report
  • [Presentation] GENERATION OF CLONED MICE BY DIRECT NUCLEAR TRANSFER FROM ADULT DIFFERENTIATED NEURAL CELLS2012

    • Author(s)
      Mizutani E., Kassai H., Wakayama T., Aiba A., Ogura A.
    • Organizer
      9th Asian Reproductive Biotechnology Society
    • Place of Presentation
      フィリピン マニラ
    • Related Report
      2012 Research-status Report
  • [Presentation] 成体マウス脳神経細胞からのクローンマウス作出2012

    • Author(s)
      水谷英二、葛西秀俊、若山照彦、饗場篤、小倉淳郎
    • Organizer
      第105回 日本繁殖生物学会
    • Place of Presentation
      つくば市 筑波大学
    • Related Report
      2012 Research-status Report
  • [Remarks] 山梨大学発生工学研究センターHP

    • URL

      http://www.ccn.yamanashi.ac.jp/~twakayama/LSHP/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 山梨大学ライフサイエンス実験施設HP

    • URL

      http://www.ccn.yamanashi.ac.jp/~twakayama/LSHP/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] クローンのクローンからまたクローン

    • URL

      http://www.cdb.riken.jp/jp/04_news/articles/13/130310_recloning.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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