Searching for prerequisites for full term development of cloned embryos based on the live cell imaging analysis
Project/Area Number |
24780277
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied animal science
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Research Institution | University of Yamanashi |
Principal Investigator |
MIZUTANI Eiji 山梨大学, 総合研究部, 助教 (80443034)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | クローン / ライブセルイメージング / 初期胚発生 / 核移植 / 成体脳神経細胞 / イメージング / 胚発生 |
Outline of Final Research Achievements |
Live-cell imaging analysis revealed that most of mouse cloned embryos and ICSI embryos with freeze-dried spermatozoa occurred abnormal chromosome segregation (ACS). We found that cloned embryos formed micro pronuclei proceeding with time after donor cell injection and most of such embryos occurred ACS in subsequent development. To find cell types with high cloning efficiency, we also sought less amount of a repressive histone mark, dimethylated histone H3 lysine 9 (H3K9m2), and identified CA1 pyramidal cells in the hippocampus and Purkinje cells in the cerebellum as candidates. Using CA1 cells, cloned offspring were obtained at high rates, reaching 10%. This is for the first time demonstrated that adult neurons could be cloned by nuclear transfer. Furthermore, our data may imply that reduced H3K9me2 and increased histone acetylation would act synergistically to improve development of cloned embryos.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Generation of cloned mice from adult neurons by direct nuclear transfer.2015
Author(s)
Mizutani E, Oikawa M, Kassai H, Inoue K, Shiura H, Hirasawa R, Kamimura S, Matoba S, Ogonuki N, Nagatomo H, Abe K, Wakayama T, Aiba A, Ogura A.
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Journal Title
Biology of Reproduction
Volume: 92(3) 81
Pages: 1-11
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] RNA sequencing-based identification of aberrant imprinting in cloned mice.2014
Author(s)
Okae H, Matoba S, Nagashima T, Mizutani E, Inoue K, Ogonuki N, Chiba H, Funayama R, Tanaka S, Yaegashi N, Nakayama K, Sasaki H, Ogura A, Arima T.
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Journal Title
Hum Mol Genet.
Volume: 23
Issue: 4
Pages: 992-1001
DOI
Related Report
Peer Reviewed
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[Journal Article] Successful Serial Recloning in the Mouse over Multiple Generations.2013
Author(s)
Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T.
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Journal Title
Cell Stem Cell
Volume: 12
Issue: 3
Pages: 293-297
DOI
Related Report
Peer Reviewed
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