Analysis of MHC class I-mediated cellular entry of herpesvirus

• Project/Area Number: 24780279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: SASAKI Michihito 北海道大学, 人獣共通感染症リサーチセンター, 研究員 (70609403)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ヘルペスウイルス / レセプター / MHCクラスI / ウマ / ウサギ / 組換えタンパク質

Outline of Final Research Achievements

In this study, we revealed that equine major histocompatibility complex (MHC) class I binds to equine herpesvirus type 4 (EHV-4) glycoprotein D and mediates the cellular entry of EHV-4. We also showed that the treatment of recombinant proteins binding to equine MHC class I inhibits the entry of both EHV-1 and EHV-4, suggesting that the blocking of equine MHC class I will be a candidate for the prevention and treatment of EHV-1 and EHV-4 infections. In addition, we analyzed the function of rabbit MHC class I in EHV-1 entry into RK13 cells.

Research Products

• [Journal Article] Metagenomic analysis of the shrew enteric virome reveals novel viruses related to human stool-associated viruses. (2015)
  • Author(s): Sasaki M, Orba Y, Ueno K, Ishii A, Moonga L, Hang'ombe BM, Mweene AS, Ito K, Sawa H.
  • Journal Title: Journal of General Virology Volume: 96 Issue: 2 Pages: 440-452
  • DOI: 10.1099/vir.0.071209-0
  • NAID: 120005697198
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a novel alphaherpesvirus in fruit bats. (2014)
  • Author(s): Sasaki M. et al.
  • Journal Title: Journal of Virology Volume: 88 Issue: 17 Pages: 9819-9829
  • DOI: 10.1128/jvi.01277-14
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of paramyxoviruses in Zambian wild rodents and shrews. (2014)
  • Author(s): Sasaki M. et al.
  • Journal Title: Journal of General Virology Volume: 95 Issue: 2 Pages: 325-330
  • DOI: 10.1099/vir.0.058404-0
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 3 in wild primates, Zambia. (2013)
  • Author(s): Sasaki M. et al.
  • Journal Title: Emerging Infectious Diseases Volume: 19 Issue: 9 Pages: 1500-1503
  • DOI: 10.3201/eid1909.121404
  • Peer Reviewed
• [Journal Article] Molecular detection of a novel paramyxovirus in fruit bats from Indonesia. (2012)
  • Author(s): Sasaki M, et al.
  • Journal Title: Virology Journal Volume: 9 Issue: 1 Pages: 240-240
  • DOI: 10.1186/1743-422x-9-240
  • Peer Reviewed
• [Presentation] Metagenomic Analysis of Shrew Fecal Viral Flora (2014)
  • Author(s): Sasaki M, Orba Y, Ueno K, Ishii A, Ito K, Sawa H
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 糞便メタゲノム解析によるジネズミ保有ウイルスの検索 (2014)
  • Author(s): 佐々木道仁、大場靖子、上野恵介、石井秋宏、伊藤公人、澤洋文
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] インドネシア共和国に生息するオオコウモリから分離した新規ヘルペスウイルスの性状解析 (2013)
  • Author(s): 佐々木道仁、Agus Setiyono、 Ekowati Handharyani、 中村一郎、澤洋文、木村享史
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜県）
• [Presentation] オオコウモリ由来新規ヘルペスウイルスの分離と性状解析 (2013)
  • Author(s): 佐々木道仁、Agus Setiyono、 Ekowati Handharyani、 中村一郎、澤洋文、木村享史
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] Novel herpesvirus in Indonesian fruit bats (2013)
  • Author(s): Sasaki M, Agus Setiyono, Ekowati Handharyani, Nakamura I, Sawa H. Kimura T
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県）
• [Presentation] Human parainfluenza virus type 3 infection in wild non-human primates, Zambia (2013)
  • Author(s): Sasaki M, Ishii A, Orba Y, Thomas Y, Hang’ombe B, Moonga L, Mweene A, Ogawa H, Nakamura I, Kimura T, Sawa H
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections
  • Place of Presentation: Tokyo Medical and Dental University（Tokyo, Japan）
• [Presentation] MHC class I-mediated entry of animal herpesvirus (2012)
  • Author(s): Sasaki M, Igarashi M, Hasebe R, Ito K, Fukushi H, Sawa H, Kimura T
  • Organizer: The 34th Naito Conference
  • Place of Presentation: Chateraise Gateaux Kingdom SAPPORO（Sapporo, Japan）
• [Presentation] 霊長類動物におけるパラインフルエンザウイルスの疫学調査 (2012)
  • Author(s): 佐々木道仁、石井秋宏、大場靖子、トーマス由佳、小川寛人、中村一郎、木村享史、澤洋文
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪）
• [Presentation] Human parainfluenza virus type 3 infection in wild primates (2012)
  • Author(s): 佐々木道仁、石井秋宏、大場靖子、トーマス由佳、小川寛人、中村一郎、木村享史、澤洋文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Remarks] 北海道大学人獣共通感染症リサーチセンター分子病態・診断部門ホームページ
  • URL: http://www.czc.hokudai.ac.jp/pathobiol/