Mechanisms of development of AIDS encephalopathy using a new transgenic mouse model expressing HIV-1 Nef

• Project/Area Number: 24780280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: TOMIOKA Yukiko 北海道大学, 遺伝子病制御研究所, 助教 (50374674)
• Research Collaborator: ONO Etsuro 九州大学, 医学研究院, 教授 (00160903) ; MORIMATSU Masami 北海道大学, 獣医学研究科, 准教授 (70241370)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 病態 / 疾患モデル / HIV-1 / Nef / エイズ脳症 / トランスジェニックマウス

Research Abstract

Transgenic mice expressing Human Immunodeficiency Virus 1 (HIV-1) accessory protein Nef (Nef Tg) showed the severe neurological symptom including motor ataxia, hindquarter paralysis, and epileptic seizure. Histopathologically, prominent astrocytosis, microgliosis, vacuolation of the white matter, lymphocytic infiltration were observed in the brain and spinal cord of Nef Tg. In addition, expression of some cytokines such as IP-10 was increased in the brain of Nef Tg. These pathological changes were similar to those of AIDS encephalopathy in human, which indicated that Nef protein plays an important role in neuropathogenesis of HIV-1 infection.

Research Products

• [Journal Article] Abnormal spermatogenesis and reduced fertility in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus (2013)
  • Author(s): Tomioka, Y., Morimatsu, M., Taharaguchi, S., Yamamoto, S., Suyama, H., Ozaki, K., Iwamori, N., Ono, E
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 440 Pages: 683-688
  • Peer Reviewed
• [Journal Article] Abnormal spermatogenesis and reduced fertility in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus (2013)
  • Author(s): Tomioka Y, Morimatsu M, Taharaguchi S, Yamamoto S, Suyama H, Ozaki K, Iwamori N, Ono E.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 440 Issue: 4 Pages: 683-688
  • DOI: 10.1016/j.bbrc.2013.09.125
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among healthy poultry flocks in farms in northern Vietnam (2012)
  • Author(s): Takakuwa, H., Yamashiro, T., Le, M.Q., Phuong, L.S., Ozaki, H., Tsunekuni, R., Usui, T., Ito, H., Morimatsu, M., Tomioka, Y., Yamaguchi, T., Ito, T., Murase, T., Ono, E., Otsuki, K
  • Journal Title: Prev. Vet. Med Volume: 103 Pages: 192-200
  • NAID: 120006985204
  • Peer Reviewed
• [Presentation] オーエスキー病ウイルス前初期蛋白質IE180発現トランスジェニックマウスにおける精子発生異常の解析
  • Author(s): 富岡 幸子
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学（東京都）