Elucidation of mechanisms for controlling stage conversion of Toxoplasma gondii

• Project/Area Number: 24780295
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Kagoshima University (2013); Obihiro University of Agriculture and Veterinary Medicine (2012)
• Principal Investigator: MASATANI Tatsunori 鹿児島大学, 獣医学部, 助教 (70614072)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: トキソプラズマ / ステージ変換 / 潜伏感染

Research Abstract

Toxoplasma gondii (T. gondii), an obligate intracellular protozoa, has ability to differentiate from a rapidly replicating tachyzoite stage (Tz) during acute infection to a dormant bradyzoite stage (Bz) contained in tissue cysts. In this study, We identified, cloned and characterized some genes increased in Bz stage. On the other hand, we found that expression levels of some interferon (IFN) -inducible anti-viral proteins, such as OAS1, Mx1 and ISG15 in Bz-infected cells were higher than those in uninfected and Tz-infected cells. Further analyses suggest that T.gondii latent infected animals and cells have resistance to viruses.

Research Products

• [Journal Article] Babesia bovis dihydroorotate dehydrogenase (BboDHODH) is a novel molecular target of drug for bovine babesiosis (2014)
  • Author(s): Ketsarin Kamyingkird, Shinuo Cao, Tatsunori Masatani, Paul F. Moumouni, Patrick Vudriko, Ahmed A. Mousa, Mohamad A. Terkawi, Yoshifumi Nishikawa, Ikuo Igarashi, Xuenan Xuan
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Pages: 323-330
  • NAID: 130003382372
  • Peer Reviewed
• [Journal Article] Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni (2014)
  • Author(s): Tatsunori Masatani, Masahito Asada, Madoka Ichikawa-Seki, Miho Usui, Mohamad A. Terkawi,Kei Hayashi, Shin-ichiro Kawazu, Xuenan Xuan
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Pages: 139-143
  • NAID: 130003362301
  • Peer Reviewed
• [Journal Article] Mycophenolic acid, mycophenolate mofetil, mizoribine, ribavirin, and 7-nitroindole inhibit propagation of Babesia parasites by targeting inosine 5'-monophosphate dehydrogenase (2014)
  • Author(s): Cao S., Aboge G. O., Terkawi M. A., Zhou M., Kamyingkird K., Adjou Moumouni P. F., Masatani T., Igarashi I., Nishikawa Y., Xuan X.
  • Journal Title: Journal of Parasitology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Cloning and Characterization of a 2-Cys Peroxiredoxin from <i>Babesia gibsoni</i> (2014)
  • Author(s): Masatani T, Asada M, Ichikawa-Seki M, Usui M, Terkawi MA, Hayashi K, Kawazu S, Xuan X.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 1 Pages: 139-143
  • DOI: 10.1292/jvms.13-0274
  • NAID: 130003362301
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Molecular characterization of a new Babesia bovisthrombospondin-related anonymous protein (BbTRAP2) (2013)
  • Author(s): Mohamad A. Terkawi, Jadsada Ratthanophart, Akram Salama, Mahmoud AbouLaila, Masahito Asada, Akio Ueno, Hend Alhasan, Azirwan Guswanto, Tatsunori Masatani, Naoaki Yokoyama, Yoshifumi Nishikawa, Xuenan Xuan, Ikuo Igarash
  • Journal Title: PLoS ONE Volume: 8
  • Peer Reviewed
• [Journal Article] Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs (2013)
  • Author(s): Shinuo Cao, Ahmed A. Mousa, Gabriel O. Aboge, Ketsarin Kamyingkird, Paul F. Moumouni, Mohamad A. Terkawi, Tatsunori Masatani, Yoshifumi Nishikawa, Hiroshi Suzuki, Shinya Fukumoto, Xuenan Xuan
  • Journal Title: Acta Parasitologica Volume: 58 Pages: 619-623
  • Peer Reviewed
• [Journal Article] Molecular characterization and antigenic properties of a novel Babesia gibsoni glutamic acid-rich protein (BgGARP) (2013)
  • Author(s): Ahmed A. Mousa, Shinuo Cao, Gabriel O. Aboge, Mohamad A. Terkawi,Ahmed E. Kirdasy, Akram Salama, Mabrouk Attia, Mahmoud Aboulaila, Mo Zhou,Ketsarin Kamyingkird, Paul F. Moumouni, Tatsunori Masatani, Sami A. Aziz, Waheed M. Moussa, Bayin Chahan, Shinya Fukumoto, Yoshifumi Nishikawa, Salah S. Ballal, Xuenan Xuan
  • Journal Title: Experimental Parasitology Volume: 135 Pages: 414-420
  • Peer Reviewed
• [Journal Article] Molecular detection of Theileria species in sheep from Northern China (2013)
  • Author(s): Shinuo Cao, Shoufa Zhang, Lijun Jia, Shujiang Xue, Longzheng Yu, Ketsarin Kamyingkird, Paul F. Moumouni, Ahmed A. Mousa, Mo Zhou, Yuanming Zhang, Mohamad A. Terkawi, Tatsunori Masatani, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Pages: 1227-1230
  • NAID: 130003362191
  • Peer Reviewed
• [Journal Article] Epidemiological survey of Babesia bovis and Babesia bigemina infections of cattle (2013)
  • Author(s): Longzheng Yu, Mohamad A. Terkawi, Mary J. Cruz-Flores, Florensia G. Claveria, Gabriel O. Aboge, Junya Yamagishi, Youn-Kyoung Goo, Shinuo Cao, Tatsunori Masatani, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Pages: 995-998
  • Peer Reviewed
• [Journal Article] Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA) (2013)
  • Author(s): Shinuo Cao, Yuzi Luo, Gabriel O. Aboge, Mohamad A. Terkawi, Tatsunori Masatani, Hiroshi Suzuki, Ikuo Igarashi, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Experimental Parasitology Volume: 133 Pages: 346-352
  • Peer Reviewed
• [Journal Article] Identification, cloning and characterization of BmP41, a common antigenic protein of Babasia microti (2013)
  • Author(s): Tatsunori Masatani, Hideo Ooka, Mohamad A. Terkawi, Cao Shinuo, Yuzi Luo, Masahito Asada, Kei Hayashi, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Pages: 967-970
  • Peer Reviewed
• [Journal Article] TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network (2013)
  • Author(s): Tatsunori Masatani, Tomohide Matsuo, Tetsuya Tanaka, Mohamad A. Terkawi, Eung-Goo Lee, Youn-Kyoung Goo, Gabriel O. Aboge, Junya Yamagishi, Kei Hayashi, Kyohko Kameyama, Shinuo Cao, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Parasitology International Volume: 62 Pages: 372-379
  • Peer Reviewed
• [Journal Article] Cloning, characterization and validation of inosine 5′-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target (2013)
  • Author(s): Shinuo Cao, Gabriel O. Aboge, Mohamad A. Terkawi, Mo Zhou, Yuzi Luo, Longzheng Yu, Yan Li, Youn-kyoung Goo, Ketsarin Kamyingkird, Tatsunori Masatani, Hiroshi Suzuki, Ikuo Igarashi, Yoshifumi Nishikawa, Xuenan Xuan
  • Journal Title: Parasitology International Volume: 62 Pages: 87-94
  • Peer Reviewed
• [Journal Article] Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs (2013)
  • Author(s): Cao S., Mousa A. A., Aboge G. O., Kamyingkird K., Zhou M., Adjou Moumouni P. F., Terkawi M. A., Masatani T., Nishikawa Y., Suzuki H., Fukumoto S., Xuan X.
  • Journal Title: Acta parasitologica Volume: 58 Issue: 4 Pages: 619-623
  • DOI: 10.2478/s11686-013-0183-1
• [Journal Article] Molecular Characterization of a New Babesia bovis Thrombospondin-Related Anonymous Protein (BbTRAP2) (2013)
  • Author(s): Terkawi, M.A., Ratthanophart, J., Salama, A., Aboulaila, M., Asada, M., Ueno, A., Alhasan, H., Guswanto, A., Masatani, T., Yokoyama, N., Nishikawa, Y., Xuan, X., and Igarashi, I
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e83305-e83305
  • DOI: 10.1371/journal.pone.0083305
  • Peer Reviewed
• [Journal Article] Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of <i>Babesia microti</i> (2013)
  • Author(s): Masatani T., Ooka H., Terkawi M. A., Cao S., Luo Y., Asada M., Hayashi K., Nishikawa Y., Xuan X.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 7 Pages: 967-970
  • DOI: 10.1292/jvms.12-0535
  • NAID: 130003362150
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Characterization of Toxoplasma gondii 5'UTR with encyclopedic TSS information (2012)
  • Author(s): Junya Yamagishi, Junichi Watanabe, Youn-Kyoung Goo, Tatsunori Masatani, Yutaka Suzuki, Xuenan Xuan
  • Journal Title: Journal of Parasitology Volume: 98 Pages: 445-447
  • Peer Reviewed
• [Journal Article] Macrophage induces cross-protective immunity conferred by Babesia microti against Babesia rodhaini infection in mice (2012)
  • Author(s): Yan Li, Mohamad A. Terkawi, Yoshifumi Nishikawa, Gabriel O. Aboge, Yuzi Luo, Hideo Ooka, Youn-Kyoung Goo, Longzheng Yu, Shinuo Cao, Yongfeng Sun, Junya Yamagishi, Tatsunori Masatani, Naoaki Yokoyama, Ikuo Igarashi, Xuenan Xuan
  • Journal Title: Infection and Immunity Volume: 80 Pages: 311-320
  • Peer Reviewed
• [Journal Article] A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model (2012)
  • Author(s): Yuzi Luo, Mohamed A. Terkawi, Honglin Jia, Gabriel O. Aboge, Youn-Kyoung Goo, Shinuo Cao, Yan Li, Longzheng Yu, Hideo Ooka, Ketsarin Kamyingkird, Tatsunori Masatani, Shoufa Zhang, Yoshifumi Nishikawa, Ikuo Igarashi, Xuenan Xuan
  • Journal Title: Experimental Parasitology Volume: 130 Pages: 178-182
  • Peer Reviewed
• [Journal Article] Characterization of Toxoplasma gondii 5' UTR with encyclopedic TSS information (2012)
  • Author(s): Yamagishi, et al
  • Journal Title: J.Parasitol. Volume: 98 Issue: 2 Pages: 455-457
  • DOI: 10.1645/ge-2864.1
  • Peer Reviewed
• [Presentation] 誘導下TgMAPK1発現原虫を用いたトキソプラズマ細胞分裂制御の解析 (2014)
  • Author(s): 杉達紀、竹前等、石和玲子、高野量、Recuenco Frances、村越ふみ、正谷達謄、堀本泰介、明石博臣、加藤健太郎
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛
  • Year and Date: 2014-03-28
• [Presentation] トキソプラズマ潜伏感染時に誘導される抗ウイルス応答のin vivo解析 (2013)
  • Author(s): 正谷達謄、林慶、山岸潤也、西川義文、玄学南
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-09-21
• [Presentation] Toxoplasma gondiiブラディゾイト虫体は宿主の抗ウイルス自然免疫応答を誘導する (2013)
  • Author(s): 正谷達謄、山岸潤也、西川義文、玄学南
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-30
• [Presentation] Toxoplasma gondiiのブラディゾイト特異的ロプトリータンパク質の解析 (2013)
  • Author(s): 林 慶、正谷達謄、山岸潤也、西川義文、玄 学南
  • Organizer: 第21回分子寄生虫学ワークショップ
  • Place of Presentation: 神戸市セミナーハウス
• [Presentation] Toxoplasma gondiiブラディゾイト虫体は宿主の抗ウイルス自然免疫応答を誘 導する (2013)
  • Author(s): 正谷達謄、山岸潤也、西川義文、玄学南
  • Organizer: 第82回日本寄生虫学会学術集会
  • Place of Presentation: 東京
• [Presentation] トキソプラズマ原虫がステージ特異的に誘導する抗ウイルス自然免疫応答 (2012)
  • Author(s): 正谷達謄、山岸潤也、西川義文、玄学南
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2012-09-16
• [Presentation] トキソプラズマ原虫のDense granuleに局在する新規蛋白質の性状解析 (2012)
  • Author(s): 正谷達謄、李應具、具潤景、山岸潤也、Terkawi M. Alaa、于龍政、林慶、亀山響子、西川義文、玄学南
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉
  • Year and Date: 2012-03-28
• [Presentation] トキソプラズマ原虫がステージ特異的に誘導する抗ウイルス自然免疫応答 (2012)
  • Author(s): 正谷達謄、山岸潤也、西川義文、玄学南
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 盛岡
• [Presentation] トキソプラズマ原虫ブラディゾイトステージ虫体が 誘導する抗ウイルス自然免疫応答 (2012)
  • Author(s): 正谷達謄、山岸潤也、西川義文、玄学南
  • Organizer: 第20回分子寄生虫学ワークショップ
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://kuris.cc.kagoshima-u.ac.jp/615942.html
• [Patent(Industrial Property Rights)] 抗原虫薬のスクリーニング方法及び組換えトキソプラズマ株 (2014)
  • Inventor(s): 加藤健太郎、杉達紀、正谷達謄
  • Industrial Property Rights Holder: 加藤健太郎、杉達紀、正谷達謄
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-27