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Novel influenza A virus proteins translated from PA mRNA

Research Project

Project/Area Number 24780296
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

MURAMOTO Yukiko  東京大学, 医科学研究所, 特任研究員 (70436567)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsインフルエンザウイルス
Research Abstract

I identified novel PA-related proteins in influenza A virus-infected cells. These proteins are translated from the eleventh and thirteenth in-frame AUG codons in the PA mRNA and are, therefore, N-terminally truncated forms of PA, which we named PA-N155 and PA-N182, respectively. The eleventh and thirteenth AUG codons are highly conserved among influenza A viruses, and the PA-N155 and PA-N182 proteins were detected in cells infected with various influenza A viruses isolated from different host species, suggesting the expression of these N-truncated PAs is universal in nature among influenza A viruses. These N-truncated PAs did not show polymerase activity when expressed together with PB1 and PB2; however, mutant viruses lacking the N-truncated PAs replicated more slowly in cell culture and had lower pathogenicity in mice than did wild-type virus. These results suggest that these novel PA-related proteins likely possess important functions in the replication cycle of influenza A virus.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results)

  • [Journal Article] The genome-packaging signal of the influenza A virus genome comprises a genome incorporation signal and a genome-bundling signal.2013

    • Author(s)
      Goto H, Muramoto Y, Noda T, Kawaoka Y.
    • Journal Title

      J Virol.

      Volume: 87 Issue: 21 Pages: 11316-11322

    • DOI

      10.1128/jvi.01301-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel influenza A virus proteins translated from PA mRNA.2013

    • Author(s)
      Muramoto Y
    • Journal Title

      J Virol

      Volume: 87 Issue: 5 Pages: 2455-62

    • DOI

      10.1128/jvi.02656-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Integrated network analysis reveals a novel role for the cell cycle in 2009 pandemic influenza virus-induced inflammation in macaque lungs2012

    • Author(s)
      Jason E Shoemaker, Satoshi Fukuyama, Amie J Eisfeld, Yukiko Muramoto, Shinji Watanabe, Tokiko Watanabe, Yukiko Matsuoka, Hiroaki Kitano and Yoshihiro Kawaoka
    • Journal Title

      BMC Systems Biology

      Volume: 6 Issue: 1 Pages: 117-117

    • DOI

      10.1186/1752-0509-6-117

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Integrated Clinical, Pathologic, Virologic, and Transcriptomic Analysis of H5N1 Influenza Virus-Induced Viral Pneumonia in the Rhesus Macaque2012

    • Author(s)
      Shinya K, Gao Y, Cilloniz C, Suzuki Y, Fujie M, Deng G, Zhu Q, Fan S, Makino A, Muramoto Y, Fukuyama S, Tamura D, Noda T, Eisfeld AJ, Katze, MG, Chen H, Kawaoka Y
    • Journal Title

      Journal of Virology

      Volume: 86 Issue: 11 Pages: 6055-6066

    • DOI

      10.1128/jvi.00365-12

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities2014

    • Author(s)
      Yukiko Muramoto
    • Organizer
      第3回感染症若手フォーラム
    • Place of Presentation
      長崎市
    • Year and Date
      2014-02-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Integrated clinical, pathologic, virologic, and transcriptomic analysis of H5N1 influenza virus-induced viral pneumonia in the rhesus macaque2014

    • Author(s)
      Yukiko Muramoto
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2014, Working Group Meeting
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2014-01-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities2014

    • Author(s)
      Yukiko Muramoto
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2014
    • Place of Presentation
      Sendai, Japan.
    • Year and Date
      2014-01-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Integrated clinical, pathologic, virologic, and transcriptomic analysis of H5N1 influenza virus-induced viral pneumonia in the rhesus macaque.2014

    • Author(s)
      Yukiko Muramoto.
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2014, Working Group Meeting
    • Place of Presentation
      仙台市、日本
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities.2014

    • Author(s)
      Yukiko Muramoto, Jason E. Shoemaker, Le Thi Quynh Mai, Yasushi Itoh, Daisuke Tamura, Ryuta Uraki, Yuko Sakai-Tagawa, Hirotaka Imai, Ryo Takano, Eiryo Kawakami, Kiyoko Okamoto, Hirohito Ishigaki, Takeshi Noda, Satoshi Fukuyama, Kazumasa Ogasawara, Hiroaki Kitano, Yoshihiro Kawaoka.
    • Organizer
      Asian-African Research Forum on Emerging and Reemerging Infections 2014
    • Place of Presentation
      仙台市、日本
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities.2014

    • Author(s)
      Yukiko Muramoto, Jason E. Shoemaker, Le Thi Quynh Mai, Yasushi Itoh, Daisuke Tamura, Ryuta Uraki, Yuko Sakai-Tagawa, Hirotaka Imai, Ryo Takano, Eiryo Kawakami, Kiyoko Okamoto, Hirohito Ishigaki, Takeshi Noda, Satoshi Fukuyama, Kazumasa Ogasawara, Hiroaki Kitano, Yoshihiro Kawaoka.
    • Organizer
      第3回感染症若手フォーラム
    • Place of Presentation
      長崎市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of novel influenza A virus proteins translated from PA mRNA2012

    • Author(s)
      Muramoto Y
    • Organizer
      The 11th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Hyogo, Japan
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of novel influenza A virus proteins translated from PA mRNA2012

    • Author(s)
      Yukiko Muramoto, Takeshi Noda, Eiryo Kawakami, Yoshihiro Kawaoka
    • Organizer
      The 11th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji, Hyogo
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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