Analysis of CDV quasispecies and establishement of the reverse genetics system

• Project/Area Number: 24780303
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: YOSHIKAWA Tomoki 国立感染症研究所, ウイルス第一部, 研究員 (20399463)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イヌジステンパーウイルス / ウイルス / 犬ジステンパー / リバースジェネティクス

Research Abstract

There was a canine distemper outbreak, which is generally known to be occurred in carnivore happened in the monkey colony in an animal quarantine. Hence to understand the pathogenesis, the sequences from the in vitro isolates and from these of clinical specimens were determined and analyzed by utilizing next-generation sequencing technique. In addition, establishment of the reverse genetics system based on the S-CDV sequence, which enable to produce the infectious particles from the plasmids were tried.

Research Products

• [Journal Article] Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus (2014)
  • Author(s): Atsushi Yamanaka
  • Journal Title: PLoS One Volume: ９ Issue: 3 Pages: e92777-e92777
  • DOI: 10.1371/journal.pone.0092777
  • Peer Reviewed / Open Access
• [Journal Article] The first identification and retrospective study of severe Fever with thrombocytopenia syndrome in Japan (2014)
  • Author(s): Takahashi T, Maeda K, Suzuki T, Ishido A, et al.
  • Journal Title: Journal of Infectious Diseases Volume: 209 Issue: 6 Pages: 816-827
  • DOI: 10.1093/infdis/jit603
  • Peer Reviewed / Open Access
• [Journal Article] Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. (2013)
  • Author(s): Sakai K, Nagata N, Ami Y, Seki F, Suzaki Y, Iwata-Yoshikawa N, Suzuki T, Fukushi S, Mizutani T, Yoshikawa T, Otsuki N, Kurane I, Komase K, Yamaguchi R, Hasegawa H, Saijo M, Takeda M, Morikawa S.
  • Journal Title: J. Virol. Volume: 87 Issue: 2 Pages: 1105-1114
  • DOI: 10.1128/jvi.02419-12
  • Peer Reviewed
• [Journal Article] Canine distemper virus associated with a lethal outbreak in monkeys can readily adapt to use human receptors. (2013)
  • Author(s): Sakai K, Yoshikawa T, Seki F, Fukushi S, Tahara M, Nagata N, Ami Y, Mizutani T, Kurane I, Yamaguchi R, Hasegawa H, Saijo M, Komase K, Morikawa S, Takeda M.
  • Journal Title: J Virol Volume: 87 Issue: 12 Pages: 7170-7175
  • DOI: 10.1128/jvi.03479-12
  • Peer Reviewed
• [Journal Article] Canine Distemper Virus Associated with a Lethal Outbreak in Monkeys Readily Adapted to Use Human Receptors (2013)
  • Author(s): Sakai, K. Yoshikawa, T. Seki, F. Fukushi, S. Tahara, M. Nagata, N. Ami, Y. Mizutani, T. Kurane, I. Yamaguchi, R. Hasegawa, H. Saijo, M. Komase, K. Morikawa, S. Takeda, M.
  • Journal Title: J Virol Volume: In press
  • Peer Reviewed
• [Journal Article] A seroepidemiologic study of Reston ebolavirus in swine in the Philippines (2012)
  • Author(s): Sayama, Y. Demetria, C. Saito, M. Azul, R. R. Taniguchi, S. Fukushi, S. Yoshikawa, T. Iizuka, I. Mizutani, T. Kurane, I. Malbas, F. F., Jr. Lupisan, S. Catbagan, D. P. Animas, S. B. et al.
  • Journal Title: BMC veterinary research Volume: 8 Pages: 82-82
  • Peer Reviewed
• [Journal Article] Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008 (2012)
  • Author(s): Sakai, K. Nagata, N. Ami, Y. Seki, F. Suzaki, Y. Iwata-Yoshikawa, N. Suzuki, T. Fukushi, S. Mizutani, T. Yoshikawa, T. Otsuki, N. Kurane, I. Komase, K. Yamaguchi, R. Hasegawa, H. et al.
  • Journal Title: J Virol Volume: 87 Pages: 1105-14
  • Peer Reviewed
• [Journal Article] Serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers (2012)
  • Author(s): Fukushi, S. Tani, H. Yoshikawa, T. Saijo, M. Morikawa, S.
  • Journal Title: Viruses Volume: 10 Pages: 2097-114
  • Peer Reviewed
• [Presentation] IPS-1-dependent innate immune response is indispensable for limiting the SARS-CoV propagation in airway epithelial cell (2012)
  • Author(s): Tomoki Yoshikawa, Shuetsu Fukushi, Chien-T K Tseng, Clarence J Peters
  • Organizer: The 9 th Japan - China International Conference of Virology
  • Place of Presentation: Hokkaido University
• [Presentation] 細胞培養痘そうワクチンの製造株であるワクチニアウイルスLC16m8の親株LC16mOの遺伝子安定性 (2012)
  • Author(s): 吉河智城、飯塚愛恵、谷英樹、福士秀悦、倉根一郎、西條政幸、森川茂
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜