Analysis of hydrogenase genes for the ecological elucidation of hydrogen-producing bacterial community in paddy field soil

• Project/Area Number: 24780318
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Boundary agriculture
• Research Institution: Nagoya University
• Principal Investigator: WATANABE Takeshi 名古屋大学, 生命農学研究科, 助教 (60547016)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 水素生成 / ヒドロゲナーゼ / 水田 / 群集構造解析 / 水田土壌 / FeFeヒドロゲナーゼ

Research Abstract

In this study, molecular biological analysis of hydA gene, encoding [FeFe]-hydrogenase related to hydrogen production, was carried out to reveal diversity and dynamics of the hydrogen-producing bacterial community in paddy field soil. This study showed inhabitant of diverse bacteria possessing [FeFe]-hydrogenase and their stable community structures in the paddy field soil. However, hydA-transcribing bacterial groups changed significantly during the anaerobic decomposition of organic materials in the soil, indicating that the hydrogen-producing bacterial groups and their hydrogen-producing activity varied depending on the soil conditions.

Research Products

• [Journal Article] Analysis of [FeFe]-hydrogenase genes for the elucidation of a hydrogen-producing bacterial community in paddy field soil (2014)
  • Author(s): Baba, R., Kimura, M., Asakawa, S., Watanabe, T.
  • Journal Title: FEMS Microbiol. Lett. Volume: 350 Issue: 2 Pages: 249-256
  • DOI: 10.1111/1574-6968.12335
  • URL: http://www.nts-book.co.jp/item/detail/summary/bio/20051225_42bk13.html
  • Peer Reviewed
• [Journal Article] 分子生態学的手法による水田土壌のメタン生成古細菌の動態と多様性に関する研究. (2013)
  • Author(s): 渡邉健史
  • Journal Title: 土肥誌 Volume: 84 Pages: 355-356
• [Journal Article] 水田からのメタン発生とメタン生成古細菌 (2013)
  • Author(s): 浅川晋・渡邉健史
  • Journal Title: 遺伝 Volume: 67 Pages: 579-585
• [Journal Article] Growth of hydrogenotrophic and acetoclastic methanogens on substrate from rice plant callus cells in anerobic soil: an estimation to the role of slough-off root cap cells to their growth (2013)
  • Author(s): Li, Y., Watanabe, T., Murase, J., Asakawa, S., Kimura, M
  • Journal Title: Soil Sci. Plant Nutr. Volume: 59 Issue: 4 Pages: 548-558
  • DOI: 10.1080/00380768.2013.802211
  • Peer Reviewed
• [Journal Article] 分子生態学的手法による水田土壌のメタン生成古細菌の動態と多様性に関する研究 (2013)
  • Author(s): 渡邉健史
  • Journal Title: 日本土壌肥料学雑誌 Volume: 84 Pages: 355-356
• [Journal Article] Changes in community structure of methanogenic archaea brought about by water-saving practice in paddy field soil (2013)
  • Author(s): Takeshi Watanabe, Yasukazu Hosen, Ruth Agbisit, Lizzida Llorca, Nobuko Katayanagi, Susumu Asakawa, Makoto Kimura
  • Journal Title: Soil Biology and Biochemistry Volume: 58 Pages: 235-243
  • DOI: 10.1016/j.soilbio.2012.11.022
  • Peer Reviewed
• [Journal Article] Growth of methanogens in an oxic soil microcosm: Elucidation by a DNA-SIP experiment using 13C-labeled dried rice callus (2012)
  • Author(s): Chol Gyu Lee, Takeshi Watanabe, Jun Murase, Susumu Asakawa, Makoto Kimura
  • Journal Title: Applied Soil Ecology Volume: 58 Pages: 37-44
  • DOI: 10.1016/j.apsoil.2012.03.002
  • Peer Reviewed
• [Journal Article] Bacterial communities that decompose root cap cells in an anaerobic soil: estimation by DNA-SIP method using rice plant callus cells (2012)
  • Author(s): Yong Li, Takeshi Watanabe, Susumu Asakawa, Makoto Kimura
  • Journal Title: Soil Science and Plant Nutrition Volume: 58 Issue: 3 Pages: 297-308
  • DOI: 10.1080/00380768.2012.698594
  • NAID: 110009580105
  • Peer Reviewed
• [Journal Article] Effect of winter-flooding on methanogenic archaeal community structure in paddy field under organic farming (2012)
  • Author(s): Dongyan Liu, Chika Suekuni, Kazunori Akita, Toyoaki Ito, Masanori Saito, Takeshi Watanabe, Makoto Kimura, Susumu Asakawa
  • Journal Title: Soil Science and Plant Nutrition Volume: 58 Issue: 5 Pages: 553-561
  • DOI: 10.1080/00380768.2012.726598
  • NAID: 110009580115
  • Peer Reviewed
• [Presentation] 生態学的手法による水田土壌のメタン生成古細菌の動態と多様性に (2013)
  • Author(s): 渡邉健史
  • Organizer: 多様性に関する研究.日本土壌肥料学会2013年度
  • Place of Presentation: 1
  • Year and Date: 2013-09-11
• [Presentation] 水田土壌の水素生成微生物群集構造解析 (2013)
  • Author(s): 馬場竜子・木村眞人・浅川晋・渡邉健史
  • Organizer: 日本土壌肥料学会2013年度名古屋大会
  • Place of Presentation: 名古屋
• [Presentation] 異化的亜硫酸還元酵素遺伝子およびホルミルテトラヒドロ葉酸合成酵素遺伝子を対象とした水田土壌の硫酸還元菌およびCO_2還元酢酸生成菌の群集構造解析 (2013)
  • Author(s): 水野かすみ・浅川晋・渡邉健史
  • Organizer: 日本土壌肥料学会2013年度名古屋大会
• [Presentation] Effects of winter flooding and paddy-upland rotation on microbial communities in paddy field soil (2013)
  • Author(s): Asakawa, S., Suekuni, C., Liu, D., Ishikawa, H., Nishida, M., Tsuchiya, K., Akita, K., Ito, T., Saito, M., Watanabe, T., Kimura, M.
  • Organizer: 11th Conference of the East and Southeast Asian Federation of Soil Science
  • Place of Presentation: インドネシア、ボゴール
• [Presentation] Bacterial consortia in iron-deposited colonies formed on paddy soil surface under microaerobic condition (2013)
  • Author(s): Watanabe, T., Sumida, H., Do, M.N., Yano, K., Asakawa, S., Kimura, M.
  • Organizer: 11th Conference of the East and Southeast Asian Federation of Soil Science
  • Place of Presentation: インドネシア、ボゴール
• [Presentation] 分子生態学的手法による水田土壌のメタン生成古細菌の動態と多様性に関する研究 (2013)
  • Author(s): 渡邉健史
  • Organizer: 日本土壌肥料学会2013年度名古屋大会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] 異化的亜硫酸還元酵素遺伝子およびホルミルテトラヒドロ葉酸合成酵素遺伝子を対象とした水田土壌の硫酸還元菌およびCO2還元酢酸生成菌の群集構造解析 (2013)
  • Author(s): 水野かすみ・浅川晋・渡邉健史
  • Organizer: 日本土壌肥料学会2013年度名古屋大会
  • Place of Presentation: 名古屋
• [Presentation] Hydrogen- producing bacterial community in paddy field soils estimated by [FeFe] hydrogenase gene (2012)
  • Author(s): Ryuko Baba・Makoto Kimura・Susumu Asakawa・Takeshi Watanabe
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋
• [Presentation] Iron-oxidizers in bacterial colonies formed on surface of paddy soil (2012)
  • Author(s): Takeshi Watanabe, Susumu Asakawa, Hiroaki Sumida, Do Minh Nhut, Katsuya Yano, Susumu Asakawa, Makoto Kimura
  • Organizer: 14th International Symposium on Microbial Ecology (ISME 14)
  • Place of Presentation: コペンハーゲン
• [Presentation] 嫌気条件下の水田土壌における植物遺体由来の炭素を取り込んだ細菌群集の解析 (2012)
  • Author(s): 李哲揆・渡邉健史・浅川晋・木村眞人
  • Organizer: 日本土壌肥料学会2012年度鳥取大会
  • Place of Presentation: 鳥取
• [Presentation] 水田土壌に形成されたコロニー中の鉄酸化細菌の分子系統学的解析 (2012)
  • Author(s): 渡邉健史・隅田裕明・M.D. Nhut・矢野勝也・浅川晋・木村眞人
  • Organizer: 日本土壌肥料学会2012年度鳥取大会
  • Place of Presentation: 鳥取
• [Presentation] Hydrogen-producing bacterial community in paddy field soils estimated by [FeFe] hydrogenase gene (2012)
  • Author(s): Ryuko Baba・Makoto Kimura・Susumu Asakawa・Takeshi Watanabe
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋
• [Book] 22-1.メタン生成菌,土壌微生物実験法第3版(日本土壌微生物学会編) (2013)
  • Author(s): 渡邉健史・浅川晋
• [Book] 土壌微生物実験法第3版, 22-1. メタン生成菌 (2013)
  • Author(s): 渡邉健史・浅川晋 (分担執筆)
  • Total Pages: 7
  • Publisher: 養賢堂
• [Book] 土壌微生物実験法第3版, 22-2. メタン酸化菌 (2013)
  • Author(s): 浅川晋・渡邉健史 (分担執筆)
  • Total Pages: 6
  • Publisher: 養賢堂