| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
For the analysis and utilization of the humus accumulate functions of the volcanic ash soil and utilization, this research was carried out. In the area where the annual average temperature is moderate or high, most of the applied plant biomass are decomposed by the higher activity of soil microorganism, but in volcanic ash soil, soil organic matter accumulate. In this research, we developed the effective extraction method of soil DNA and RNA from soil, in which those were hardly extractable. And a high-labeled plants by stable isotope (13C and 15N) were grown in the closed chamber, the organic matter decomposition process by soil microorganisms in volcanic ash soil was traced by the LC / MS analysis of amino acids or organic acid. Even 90% of the stable isotope labeled organic matter was limited for about the compounds which contained from 1 to 5 carbons to the identification from the unlabeled organic matter. It was necessary higher isotope labeling for the analysis of humic process.
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