Research Project
Grant-in-Aid for Young Scientists (B)
The aim of this study is to reconstruct ionotropic receptors (IRs) of insects in Sf21 cells to demonstrate that the cell lines are available as detection elements of an odorant sensor. We introduced IR gene and co-receptor (Ir8a) gene along with calcium sensitive fluorescent protein gene into Sf21 cells to establish the cell lines that responded to the general odorant by increasing fluorescent intensity. We found that the established cell lines selectively and dose-dependently detected general odorants as fluorescent intensity change. Taken together, we successfully reconstructed the function of IR in Sf21 cells and demonstrated that the Sf21 cell lines expressing IR are available as the odorant sensor elements.
All 2015 2014 2013 2012 Other
All Presentation (12 results) (of which Invited: 4 results) Book (5 results)
