| Project/Area Number |
24780331
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 熱ショック転写因子 / ストレス応答機構 / シロイヌナズナ / タンパク質間相互作用 |
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| Research Abstract |
Arabidopsis heat shock transcription factor (Hsf) A1d and A1e regulate the expression of HsfA2 which plays an important role in responses and adaptations to stress. Elucidation of activation system of HsfA1d and A1e proteins will help to clarify the stress response system of plants. In this study, I tried the isolation and identification of HsfA1d-interacting protein by the cytosolic yeast two-hybrid system, which is based on the split-ubiquitin technique. However, I did not identify them. Therefore, I attempted to isolate the HsfA1d-interacting protein by Escherichia coli bimolecular fluorescence complementation. As a result, many positive clones were obtained. It has been reported that some of these clones were involved in thermotolerance.
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