Detection of the reactive oxygen species generated from the photo-irradiation of protoporphyrin IX

• Project/Area Number: 24790006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical pharmacy
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MATSUMURA Yuriko 東京工業大学, 生命理工学研究科, 特任講師 (10439507)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 光線力学的療法 / 活性酸素 / 一重項酸素 / ESR-スピントラッピング法

Outline of Final Research Achievements

In the present study, it was clarified that the super oxide anion radical and carbon radicals were generated with the irradiation of LED light to the Protoporphyrin IX as the photo-sensitizer in addition to the singlet oxygen. it is suggested that the carbon radicals were gnenerated from the oxidation of PpIX by the singlet oxygen. Hydroxyl radicals was generated from the LED irradiation to the cells which contain the endogenous PpIX induced by 5-aminolevulinic acid (ALA).

Research Products

• [Presentation] ESR－スピントラッピング法を用いた活性酸素計測 (2015)
  • Author(s): 松村有里子
  • Organizer: 第１回OCU物質科学フロンティアシンポジウム
  • Place of Presentation: 大阪市立大学 理学部会議室
  • Year and Date: 2015-01-09
  • Invited
• [Presentation] 5-アミノレブリン酸の培養細胞に及ぼす影響について (2014)
  • Author(s): 松村有里子・岩澤篤郎・小澤俊彦・ 河野雅弘
  • Organizer: 第１８階ESRフォーラム研究会
  • Place of Presentation: 名古屋大学 シンポジオン会議室
  • Year and Date: 2014-07-26
• [Presentation] ALA共存下で培養したガン細胞から 生成する活性酸素の研究 (2014)
  • Author(s): 松村有里子・岩澤篤郎・小澤俊彦・ 河野雅弘
  • Organizer: 第４回ポルフィリン・ALA学会年会
  • Place of Presentation: ニチイ学館・神戸ポートアイランドセンター
  • Year and Date: 2014-04-26
• [Presentation] Analysis of the reactive oxygen species generated by the near ultraviolet LED light irradiation (2014)
  • Author(s): Yuriko Matsumura, Atsuo Iwasawa, Toshihiko Ozawa, Masahiro Kohno
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International
  • Place of Presentation: 京都国際会議場
• [Presentation] Detection of the reactive oxygen species generated from the photo- irradiation of protoporphyrin IX (2013)
  • Author(s): Yuriko Matsumura, Atsuo Iwasawa, Toshihiko Ozawa, Masahiro Kohno
  • Organizer: 1st International ALA and Porphyrin Symposium
  • Place of Presentation: バーレーン
• [Presentation] Detection of the reactive oxygen species generated from the photo-irradiation of the photosensitizer used in the photodynamic therapy (2012)
  • Author(s): Yuriko Matsumura, Atsuo Iwasawa, Toshiaki Kamachi, Toshihiko Ozawaa, Masahiro Kohno
  • Organizer: 19th International SPACC Symposium
  • Place of Presentation: 北海道大学