| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The generation of antibodies specific to EGCg and rare derivatives would be useful for immunological detection of subcellular and tissue localization. Furthermore, enzyme-linked immunosorbent assays (ELISA) with color or fluorescence endpoints would be useful for quantitating trace amounts of EGCg derivatives. An efficient synthesis of 6-(5-aminopentyl)-5,7-deoxyepigallalo-EGCG (APDOEGCg) as probe precursor was accomplished by the Suzuki-Miyaura coupling, Mitsunobu reaction with our Ns (nitrobenzensulfonyl) amide, and the construction of the dihydrobenzopyran ring. The reactive amine group of APDOEGCg was readily connect to career protein without the need for phenol protection. This hapten enabled the generation of EGCg antibodies. On the other hand, the synthesis of TF analogue which possess a reactive aminoalkyl side chain was accomplished by using biomimetic dimerization with Ns protective method. It would be readily converted into hapten probe.
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