Synthesis of novel fluorescent compounds possessing a ON/OFF switching system, and their application to fluorescent probes
| Project/Area Number |
24790029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical pharmacy
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
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| Research Collaborator |
MIYASHITA Kazuyuki
OBIKA Satoshi
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 蛍光分子 / 複素環 / 緑色蛍光タンパク質 / イミダゾリノン / コレステロール / 蛍光物質 / 蛍光プローブ / GFP |
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| Outline of Final Research Achievements |
I obtained the following results in the research on the development of a novel fluorescent small molecule, namely, diarylmethyleneimidazolinone (DAIN) that mimics a green fluorescent protein chromophore.
1) Four DAIN analogs having different diaryl moieties were synthesized. 2) Two DAIN analogs equipping a side chain containing an azide group at the end were synthesized, aiming to the development of a DNA probe that can recognize the DNA sequence. 3) A potassium ion probe of DAIN was synthesized. 4) A cholesten-hybrid DAIN analog was synthesized, aiming to the application to a probe for the membrane fluidity.
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Report
(4 results)
Research Products
(5 results)
