Establishment of innovative angiognenic therapy using with in vivo imaging of angiogenesis
| Project/Area Number |
24790038
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
HAMADA Yoh 東北大学, 医学(系)研究科(研究院), 助教 (20611958)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 血管新生治療 / 血管新生メカニズム / 血管新生療法 / 血管内皮成長因子 / 血小板由来成長因子 / 血管新生 / 末梢動脈疾患 / 生体内イメージング |
|---|
| Outline of Final Research Achievements |
To establish double imaging technique which can visualize vascular endothelial and mural cells together, we intend to make novel hetero-tagged recombinant VEGF and PDGF. Finally, we succeeded in establishing these proteins. At first, we checked these normal physiological activity. Cells which expressed these receptors snowed significantly high fluorescence with protein-fluorescent particle complex.
Then, we tried to visualize vascular endothelial and mural cells simultaneously using with animal model. But, there were several difficulty such as auto-fluorescence. It took too much time to decide adequate imaging condition. Moreover, we have much trouble to make virus particles for angiogenic therapy. So, we could not accomplish our aim enough. We want to continue making effort to overcome several problems which we confront.
|
Report
(4 results)
Research Products
(4 results)
