| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To establish double imaging technique which can visualize vascular endothelial and mural cells together, we intend to make novel hetero-tagged recombinant VEGF and PDGF. Finally, we succeeded in establishing these proteins. At first, we checked these normal physiological activity. Cells which expressed these receptors snowed significantly high fluorescence with protein-fluorescent particle complex.
Then, we tried to visualize vascular endothelial and mural cells simultaneously using with animal model. But, there were several difficulty such as auto-fluorescence. It took too much time to decide adequate imaging condition. Moreover, we have much trouble to make virus particles for angiogenic therapy. So, we could not accomplish our aim enough. We want to continue making effort to overcome several problems which we confront.
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