Mechanism of cell adhesion due to lectin-glycolipid interaction

• Project/Area Number: 24790039
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: HIRAMATSU HIROTSUGU 東北大学, 薬学研究科(研究院), 助教 (90419995)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 細胞接着 / 糖鎖 / ラマン分光法 / 蛍光分光法 / レクチン / 紫外共鳴ラマン / 糖結合 / pH依存性

Research Abstract

Human galectin-1 (hGal-1) dimer is involved in cell adhesion by binding multiple saccharide moieties on surfaces of membranes. We analyzed the binding of hGal-1 to ganglioside GM1 in POPC liposome by monitoring the fluorescence of Trp68 of hGal-1, and found a marked difference of the binding constant and Hill coefficient between the binding to GM1 and a soluble saccharide lactose. The results imply that hGal-1 has different properties in binding to soluble and membrane-bound sugars.

Research Products

• [Journal Article] Vertical flow apparatus for enhancement and efficient collection of Raman signal (2014)
  • Author(s): Hirotsugu Hiramatsu and Takahiro Saito
  • Journal Title: J. Raman Spectrosc Volume: 45 Issue: 2 Pages: 208-210
  • DOI: 10.1002/jrs.4437
  • Peer Reviewed
• [Journal Article] Involvement of Histidine Residues in the pH-Dependent β-Galactoside Binding Activity of Human Galectin-1 (2013)
  • Author(s): Hirotsugu Hiramatsu, Katsuyuki Takeuchi, and Hideo Takeuchi
  • Journal Title: Biochemistry Volume: 52 Issue: 13 Pages: 2371-2380
  • DOI: 10.1021/bi4001112
  • Peer Reviewed
• [Journal Article] β-Galactoside-binding Activity of Human Galectin-1 at Basic pH (2013)
  • Author(s): Hirotsugu Hiramatsu, Katsuyuki Takeuchi, Koki Fukuda, Tomohide Nishino
  • Journal Title: Chemical Physics Volume: 419 Pages: 113-117
  • DOI: 10.1016/j.chemphys.2013.01.018
  • Peer Reviewed
• [Presentation] リポソーム表面のガングリオシドGM1糖鎖に対するヒトガレクチン1の結合 (2013)
  • Author(s): 堀良太、平松弘嗣
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-10-20
• [Presentation] 酸性および塩基性条件でのヒトガレクチン1糖結合能低下の原因 (2013)
  • Author(s): 平松弘嗣、竹内勝之、福田洸希、西野伴英、竹内英夫
  • Organizer: 生体分子科学討論会2013
  • Place of Presentation: 大阪
  • Year and Date: 2013-06-08
• [Presentation] Involvement of a Few Amino Acid Residues in the pH-Dependentβ-Galactoside-binding Activity of Human Galectin-1 (2013)
  • Author(s): Hirotsugu Hiramatsu, Katsuyuki Takeuchi, Koki Fukuda, Tomohide Nishino, Hideo Takeuchi
  • Organizer: Seventh International Conference on Advanced Vibrational Spectroscopy (ICAVS-7)
  • Place of Presentation: Kobe
• [Presentation] Involvement of a Few Amino Acid Residues in the pH-Dependent β-Galactoside-binding Activity of Human Galectin-1 (2013)
  • Author(s): Hirotsugu Hiramatsu, Katsuyuki Takeuchi, Koki Fukuda, Tomohide Nishino, Hideo Takeuchi
  • Organizer: Seventh International Conference on Advanced Vibrational Spectroscopy (ICAVS-7)
  • Place of Presentation: 神戸
• [Presentation] β-Galactoside-binding activity of human galectin-1 at basic pH (2012)
  • Author(s): Hirotsugu Hiramatsu, Tomohide Nishino, Hideo Takeuchi
  • Organizer: 第50会日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-23
• [Presentation] 酸性および塩基性条件でのヒトガレクチン１糖結合能低下の原因
  • Author(s): 平松弘嗣、竹内勝之、福田洸希、西野伴英、竹内英夫
  • Organizer: 生体分子科学討論会2013
  • Place of Presentation: 大阪
• [Presentation] リポソーム表面のガングリオシド GM1 糖鎖に対するヒトガレクチン 1 の結合
  • Author(s): 堀良太、平松弘嗣
  • Organizer: 第５２回日本薬学会東北支部大会
  • Place of Presentation: 仙台
• [Presentation] β-Galactoside-binding activity of human galectin-1 at basic pH
  • Author(s): H. Hiramatsu, T. Nishino, H. Takeuchi
  • Organizer: 第50会日本生物物理学会年会
  • Place of Presentation: 名古屋