Mechanism of cell adhesion due to lectin-glycolipid interaction
| Project/Area Number |
24790039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 細胞接着 / 糖鎖 / ラマン分光法 / 蛍光分光法 / レクチン / 紫外共鳴ラマン / 糖結合 / pH依存性 |
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| Research Abstract |
Human galectin-1 (hGal-1) dimer is involved in cell adhesion by binding multiple saccharide moieties on surfaces of membranes. We analyzed the binding of hGal-1 to ganglioside GM1 in POPC liposome by monitoring the fluorescence of Trp68 of hGal-1, and found a marked difference of the binding constant and Hill coefficient between the binding to GM1 and a soluble saccharide lactose. The results imply that hGal-1 has different properties in binding to soluble and membrane-bound sugars.
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Report
(3 results)
Research Products
(11 results)
