| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
ATP synthase generate ATP using energy from a proton motive force across the membrane. Their mechanism includes subunits rotation, which are essential for energy coupling between proton transport and catalysis. We have studied the inhibitory effects and inhibition mechanism of phytopolyphenols. I found curcumin and its analogues inhibited ATP synthase F1 sector. We also found piceatannol and beta-gamma interface mutation affect the rate-limiting transition step, even though they perturb physically separated rotor-stator interactions. These data indicate that both rotor-stator interaction sites contribute to formation of the rate limiting transition state.
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