Screening for ligands of G protein-coupled receptors using the newly developed TGFalpha shedding assay
| Project/Area Number |
24790064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
INOUE Asuka 東北大学, 薬学研究科(研究院), 助手 (50525813)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Gタンパク質共役型受容体 / GPCR / TGFalpha / リゾリン脂質 / リゾホスファチジルセリン / CRISPR / HEK293 / エクトドメイン切断 / TRPチャネル / TGFα / オーファン / P2Y10 / GPR174 |
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| Research Abstract |
In this KAKENHI project, by employing the newly developed transforming growth factor-alpha (TGFalpha) shedding assay, I screened for G protein-coupled receptors (GPCRs) that respond to bioactive molecules and developed an assay system that can quantify coupling efficiency of heterotrimeric G proteins. I identified three orphan GPCRs (P2Y10, GPR174, A630033H20) as lysophoshadylserine-specific receptors, one GPCR as a lysophoshadylglucoside receptor and two GPCRs as oxidized phospholipid receptors. I generated G protein-deficient HEK293 cells using the CRISPR-Cas9 system and successfully applied them to analyze G protein coupling of GPCRs.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo2014
Author(s)
Nakanaga K, Hama K, Kano K, Sato T, Yukiura H, Inoue A, Saigusa D, Tokuyama H, Tomioka Y, Nishina H, Kawahara A, Aoki J
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Journal Title
J. Biochem
Volume: 155
Issue: 4
Pages: 235-241
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mitochondria-type GPAT is required for mitochondrial fusion2013
Author(s)
Yohsuke Ohba, Takeshi Sakuragi, Eriko Kage-Nakadai, Naoko H Tomioka, Nozomu Kono, Rieko Imae, Asuka Inoue, Junken Aoki, Naotada Ishihara, Takao Inoue, Shohei Mitani and Hiroyuki Arai
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Journal Title
The EMBO Journal
Volume: (in press)
Issue: 9
Pages: 1265-1279
DOI
Related Report
Peer Reviewed / Open Access
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