Mechanism of regulation of gene expression by polyamines at the level of translation depending on the interaction of polyamines with RNA

• Project/Area Number: 24790084
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Chiba Institute of Science
• Principal Investigator: TERUI YUSUKE 千葉科学大学, 薬学部, 講師 (60433687)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ポリアミン / 細胞増殖 / 細胞生存率 / バイオフィルム / 大腸菌 / プトレスシン / スペルミジン / 翻訳 / スペルミン / eEF1A / Shine-Dalgarno配列 / 生存率

Research Abstract

Polyamines are essential for normal cell growth and cell viability. Polyamines exist mostly as polyamine-RNA complexes in cells. A group of genes whose expression is enhanced by polyamines at the level of translation be referred to as a "polyamine modulon".
Since biofilm formation is also involved in cell viability, we looked for proteins involved in biofilm formation and cell viability whose synthesis is stimulated by polyamines at the level of translation in Escherichia coli. It was found that the synthesis of UvrY, CpxR and RRF was increased by polyamines at the level of translation. The results indicate that polyamines are necessary for both biofilm formation and cell viability.

Research Products

• [Journal Article] Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli. (2014)
  • Author(s): Terui, Y. et al.
  • Journal Title: Amino Acids Volume: 46 Issue: 3 Pages: 661-670
  • DOI: 10.1007/s00726-013-1517-x
  • Peer Reviewed
• [Journal Article] Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle (2013)
  • Author(s): YAMASHITA Tomoko, NISHIMURA Kazuhiro, SAIKI Ryotaro, OKUDAIRA Hiroyuki, TOME Mayuko, HIGASHI Kyohei, NAKAMURA Mizuho, TERUI Yusuke, FUJIWARA Kunio, KASHIWAGI Keiko, IGARASHI Kazuei
  • Journal Title: International J ournal of Biochemistry and Cell Biology Volume: Vol 45 Pages: 1042-1050
  • Peer Reviewed
• [Journal Article] Spermidine is required for morphogenesis in the human pathogenic fungus, Penicillium marneffei (2013)
  • Author(s): KUMMASOOK Aksarakorn, COOPER Chester R. Jr., SAKAMOTO Akihiko, TERUI Yusuke, KASHIWAGI Keiko, VANITTANAKORN Nongnuch
  • Journal Title: Fungal Genetics and Biology Volume: vol 58-59 Pages: 25-32
  • Peer Reviewed
• [Journal Article] Changes in polyamines levels in Xenopus early embryogenesis (2013)
  • Author(s): Shiokawa K., Mishima T., Yoshida H., Takai J., Tsuboshima K., Soutome T., Terui Y., Abe H., Sakurai S., Takahashi T., Tashiro K., Igarashi K.
  • Journal Title: Trends in Developmental Biology Volume: 7 Pages: 25-31
  • Peer Reviewed
• [Journal Article] Spermidine is required for morphogenesis in the human pathogenic fungus, Penicillium marneffei. (2013)
  • Author(s): Kummasook, A., Cooper, C. R. Jr., Sakamoto, A., Terui, Y., Kashiwagi, K., and Vanittanakorn, N.
  • Journal Title: Fungal Genet. Biol. Volume: 58-59 Pages: 25-32
  • DOI: 10.1016/j.fgb.2013.08.001
  • Peer Reviewed
• [Journal Article] Inactivation of GAPDH as one mechanism of acrolein toxicity. (2013)
  • Author(s): Nakamura, M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 430 Issue: 4 Pages: 1265-1271
  • DOI: 10.1016/j.bbrc.2012.12.057
  • Peer Reviewed
• [Journal Article] Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle. (2013)
  • Author(s): Yamashita, T. et al.
  • Journal Title: Int. J. Biochem, Cell Biol. Volume: 45 Issue: 6 Pages: 1042-1050
  • DOI: 10.1016/j.biocel.2013.02.021
  • Peer Reviewed
• [Journal Article] Synthesis and evaluation of pyrrole polyamide-2’-deoxyguanosine 5’-phosphate hybrid. (2013)
  • Author(s): Kawashima, E., Nakanishi, Y., Terui, Y., Tomitori, H., Kashiwagi, K., Ohba, Y., and Kamaike, K.
  • Journal Title: Nucleosides, Nucleotides Nucleic Acids Volume: 32 Issue: 4 Pages: 196-205
  • DOI: 10.1080/15257770.2013.774014
  • Peer Reviewed
• [Journal Article] Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor (2012)
  • Author(s): SAKAMOTO Akihiko, TERUI Yusuke, YAMAMOTO Taku, KASAHARA Takuma, NAKAMURA Mizuho, TOMITORI Hideyuki, YAMAMOTO Kaneyoshi, MICHAEL Anthony J., IGARASHI Kazuei, KASHIWAGI Keiko
  • Journal Title: International J ournal of Biochemistry and Cell Biology Volume: vol 44 Pages: 1877-1886
  • Peer Reviewed
• [Journal Article] Increase in cell viability by polyamines through stimulation of the synthesis of ppGpp regulatory protein andωprotein of RNA polymerase in Escherichia coli (2012)
  • Author(s): TERUI Yusuke, AKIYAMA Mariko, SAKAMOTO Akihiko, TOMITORI Hideyuki, YAMAMOTO Kaneyoshi, ISHIHAMA Akira, IGARASHI Kazuei, KASHIWAGI Keiko
  • Journal Title: International J ournal of Biochemistry and Cell Biology Volume: vol 44 Pages: 412-422
  • Peer Reviewed
• [Journal Article] Increase in cell viability by polyamines through stimulation of the synthesis of ppGpp regulatory protein and ω protein of RNA polymerase in Escherichia coli (2012)
  • Author(s): Terui, Y., et al
  • Journal Title: Int.J.Biochem.Cell.Biol. Volume: 44 Issue: 2 Pages: 412-422
  • DOI: 10.1016/j.biocel.2011.11.017
  • Peer Reviewed
• [Journal Article] Augmented glutathione synthesis decreases acrolein toxicity (2012)
  • Author(s): Tomitori, H., et al
  • Journal Title: Biochem.Biophys Res.Commun. Volume: 418 Issue: 1 Pages: 110-115
  • DOI: 10.1016/j.bbrc.2011.12.143
  • Peer Reviewed
• [Journal Article] Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor. (2012)
  • Author(s): Sakamoto, A.
  • Journal Title: Int. J. Biochem. Cell Biol. Volume: 44 Issue: 11 Pages: 1877-1886
  • DOI: 10.1016/j.biocel.2012.07.010
  • Peer Reviewed
• [Presentation] Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle (2013)
  • Author(s): KASHIWAGI Keiko, Yamashita Tomoko, NISHIMURA Kazuhiro, SAIKI Ryotaro, OKUDAIRA Hiroyuki, TOME Mayuko, HIGAHI Kyohei, NAKAMURA Mizuho, TERUI Yusuke, FUJIWARA Kunio, IGARASHI Kazuei
  • Place of Presentation: Waterville Valley、USA
  • Year and Date: 2013-06-16
• [Presentation] 細胞周期進行におけるポリアミンの機能解析 (2013)
  • Author(s): 山下智子、西村和洋、斎木遼太郎、奥平宏之、當銘真悠子、東恭平、照井祐介、藤原邦雄、柏木敬子、五十嵐一衛
  • Organizer: 日本ポリアミン学会第4回大会
  • Place of Presentation: 松島・大観荘
  • Year and Date: 2013-01-25
• [Presentation] Enhanced cell viability by polyamines under oxidative stress through polyamine stimulation of SoxR and EmrR in Escherichia coli. (2013)
  • Author(s): Yusuke Terui, Akihiko Sakamoto, Akito Tamada, Tomoaki Ichihara, Taku Yamamoto, Kaneyoshi Yamamoto, Akira Ishihama, Kazuei Igarashi, Keiko Kashiwagi
  • Organizer: 2013 Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
• [Presentation] Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle. (2013)
  • Author(s): Keiko Kashiwagi, Tomoko Yamashita, Kazuhiro Nishimura, Ryotaro Saiki, Hiroyuki Okudaira, Mayuko Tome, Kyohei Higashi, Mizuho Nakamura, Yusuke Terui, Kunio Fujiwara, Kazuei Igarashi
  • Organizer: 2013 Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
• [Presentation] Polyamine stimulation of eEF1A synthesis based on the unusual position of an SD-like sequence of eEF1A mRNA (2013)
  • Author(s): Akihiko Sakamoto, Yusuke Terui, Takuma Kasahara, Taketo Yoshida, Hideyuki Tomitori, Kazuei Igarashi, Keiko Kashiwagi
  • Organizer: 2013 Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
• [Presentation] ポリアミンによるバイオフィルム形成能及び生存率の上昇 (2012)
  • Author(s): 坂本明彦、照井祐介、山本拓、笠原拓馬、富取秀行、山本兼由、石浜明、五十嵐一衛、柏木敬子
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2012-12-15
• [Presentation] Enhanced biofilm formation and cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor (2012)
  • Author(s): SAKAMOTO Akihiko, TERUI Yusuke, YAMAMOTO Taku, KASAHARA Takuma, NAKAMURA Mizuho, TOMITORI Hideyuki, YAMAMOTO Kaneyoshi, MICHAEL Anthony J., IGARASHI Kazuei, KASHIWAGI Keiko
  • Organizer: International congress on polyamines
  • Place of Presentation: Istanbul Kultur University、Turkey
  • Year and Date: 2012-09-02
• [Presentation] ポリアミンによる大腸菌二成分制御系蛋白質UvrY及びCpxR合成促進に基づくバイオフィルム形成上昇 (2012)
  • Author(s): 坂本明彦、照井祐介、山本拓、笠原拓馬、富取秀行、山本兼由、石浜明、五十嵐一衛、柏木敬子
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-03-29
• [Presentation] Identification and its effect on cell growth of acrolein-conjugated protein in mouse mammary carcinoma FM3A cells. (2012)
  • Author(s): Tomitori, H., Nakamura, M., Suzuki, T., Terui, Y., Yoshida, M., Saiki, R., Dohmae, N., Igarashi, K., Kashiwagi, K.
  • Organizer: International Congress on “Polyamines: Biological and Clinical Perspectives”
  • Place of Presentation: Istanbul Kultur University, Istanbul, Turkey
• [Presentation] Enhanced biofilm formation and cell viability by polyamines through polyamine stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor. (2012)
  • Author(s): Sakamoto, A., Terui, Y., Yamamoto, T., Kasahara, T., Tomitori, H., Yamamoto, K., Ishihama, A., Michael, A. J., Igarashi, K., Kashiwagi, K.
  • Organizer: International Congress on “Polyamines: Biological and Clinical Perspectives”
  • Place of Presentation: Istanbul Kultur University, Istanbul, Turkey
• [Presentation] Comparison of putrescine uptake catalyzed by PotFGHI and PuuP in Escherichia coli. (2012)
  • Author(s): Terui, Y., Saroj, S. D., Sakamoto, A., Yoshida, T., Higashi, K., Saiki, R., Kurihara, S., Suzuki, H., Toida, T., Kashiwagi, K., Igarashi, K.
  • Organizer: International Congress on “Polyamines: Biological and Clinical Perspectives”
  • Place of Presentation: Istanbul Kultur University, Istanbul, Turkey
• [Presentation] マウス神経芽細胞腫Neuro2a由来の新規アクロレイン耐性細胞の性質
  • Author(s): 中村瑞穂，坂本明彦，照井祐介，富取秀行，斎木遼太郎，吉田円，五十嵐一衛，柏木敬子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 酸化ストレス下における大腸菌のポリアミンの生存率維持の役割
  • Author(s): 照井祐介，坂本明彦，玉田朗人，市原朋昌，山本拓，山本兼由，石浜明，五十嵐一衛，柏木敬子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ポリアミン過剰蓄積による細胞増殖阻害機構の解明
  • Author(s): 坂本明彦，照井祐介，吉田健人，山本兼由，石浜明，五十嵐一衛，柏木敬子
  • Organizer: ポリアミンと核酸の共進化」第12回合同シンポジウム
  • Place of Presentation: 東京慈恵会医科大学 高木2号館 南講堂
• [Presentation] 酸化ストレス下におけるポリアミンモジュロンの生理的役割解明
  • Author(s): 山本拓，吉田健人，照井祐介，坂本明彦，山本兼由，石浜明，鈴木秀之，五十嵐一衛，柏木敬子
  • Organizer: 日本ポリアミン学会第5回年会
  • Place of Presentation: 千葉科学大学マリーナキャンパス
• [Presentation] アクロレイン耐性細胞Neuro2a-ATD-2の毒性解除機構の解明
  • Author(s): 中村瑞穂，坂本明彦，照井祐介，富取秀行，斎木遼太郎，吉田円，五十嵐一衛，柏木敬子
  • Organizer: 日本ポリアミン学会第5回年会
  • Place of Presentation: 千葉科学大学マリーナキャンパス
• [Presentation] サメ肌抗菌シートによる緑膿菌のバイオフィルム形成及びswarming motilityの抑制
  • Author(s): 坂本明彦，照井祐介，堀江千紘，福井貴史，増澤俊幸，菅原申太郎，重田核，重田龍男，五十嵐一衛，柏木敬子
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市総合体育館
• [Presentation] 酸化ストレス下におけるポリアミンの細胞増殖及び生存率維持に果たす役割
  • Author(s): 吉田健人，坂本明彦，照井祐介，山本拓，山本兼由，石浜明，鈴木秀之，五十嵐一衛，柏木敬子
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学工学部2号館
• [Presentation] 真核細胞における新規ポリアミンモジュロンの同定及び蛋白質合成促進機構の解明.
  • Author(s): 吉田健人, 照井祐介, 笠原拓馬, 坂本明彦, 永池 誠, 富取秀行, 五十嵐一衛, 柏木敬子
  • Organizer: ポリアミンと核酸の共進化」第11回合同シンポジウム
  • Place of Presentation: 東京慈恵会医科大学 西新橋校 西講堂
• [Presentation] アクロレイン抱合GAPDHを介したアクロレインの細胞毒性機序.
  • Author(s): 中村瑞穂, 富取秀行, 鈴木健裕, 照井祐介, 斎木遼太郎, 堂前 直, 五十嵐一衛, 柏木敬子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] ポリアミンによるバイオフィルム形成能及び生存率の上昇.
  • Author(s): 坂本明彦, 照井祐介, 山本 拓, 笠原拓馬, 富取秀行, 山本兼由, 石浜 明, 五十嵐一衛, 柏木敬子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] 翻訳因子eIF1Aのポリアミンによる蛋白質合成促進機構の解明.
  • Author(s): 笠原拓馬, 照井祐介, 吉田健人, 坂本明彦, 富取秀行, 五十嵐一衛, 柏木敬子
  • Organizer: 日本ポリアミン学会第4回大会
  • Place of Presentation: 松島・ホテル大観荘
• [Presentation] 細胞周期進行におけるポリアミンの機能解析.
  • Author(s): 山下智子, 西村和洋, 斎木遼太郎, 奥平宏之, 當銘真悠子, 東 恭平, 照井祐介, 藤原邦雄, 柏木敬子, 五十嵐一衛
  • Organizer: 日本ポリアミン学会第4回大会
  • Place of Presentation: 松島・ホテル大観荘
• [Presentation] GAPDHを介したアクロレイン毒性機序の解明.
  • Author(s): 中村瑞穂, 富取秀行, 鈴木健裕, 照井祐介, 斎木遼太郎, 堂前 直, 五十嵐一衛, 柏木敬子
  • Organizer: 日本ポリアミン学会第4回大会
  • Place of Presentation: 松島・ホテル大観荘
• [Presentation] ポリアミンによる翻訳伸長因子eIF1Aの蛋白質合成促進機構の解明.
  • Author(s): 坂本明彦, 照井祐介, 吉田健人, 笠原拓馬, 富取秀行, 五十嵐一衛, 柏木敬子
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 大腸菌プトレスシン輸送蛋白質PotFGHI及びPuuPの生理的意義の解明.
  • Author(s): 照井祐介, Sunil D. Saroj, 坂本明彦, 吉田健人, 東 恭平, 斎木遼太郎, 栗原 新, 鈴木秀之, 戸井田敏彦, 柏木敬子, 五十嵐一衛
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.cis.ac.jp/~kyoin_info/PP/yterui.html