Study of physiological functions of D-amino acid degradative enzymes: role of D-amino acids in reproduction and neurotransmission
| Project/Area Number |
24790086
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | D-アミノ酸 / D-アミノ酸オキシダーゼ / D-アスパラギン酸オキシダーゼ / 線虫 / セロトニン / 寿命 / トリプトファン / 欠損変異株 / D-アスパラギン酸 / D-セリン / D-アラニン / D-グルタミン酸 |
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| Research Abstract |
Caenorhabditis elegans, a model organism has four genes that encode the enzymes degrading D-amino acids oxidatively. One of them encodes D-amino acid oxidase, which degrades neutral and basic D-amino acids, while the other three are D-aspartate oxidases, which degrade acidic D-amino acids. In the mutants defective in each gene, several phenotypic changes are observed such as decreased brood size, along with an increase in D-amino acid contents in the body. In this work, the quality of germ cells and the neurotransmission in the mutants were investigated and compared with those in the wild-type strain. In addition, several genes those expressions are altered in the mutants were identified by DNA microarray analysis, and subsequently the contents of metabolites in the metabolic pathway involved in the identified genes were determined and compared with those of the wild-type. Based on these results, the physiological roles of D-amino acids and their degradative enzymes were discussed.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] The antiviral drug acyclovir is a slow-binding inhibitor of D-amino acid oxidase2013
Author(s)
M. Katane, S. Matsuda, Y. Saitoh, M. Sekine, T. Furuchi, N. Koyama, I. Nakagome, H. Tomoda, S. Hirono, and H. Homma
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Journal Title
Biochemistry
Volume: 52, (33)
Issue: 33
Pages: 5665-5674
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of novel D-amino acid oxidase inhibitors by in silico screening and their functional characterization in vitro2013
Author(s)
M. Katane, N. Osaka, S. Matsuda, K. Maeda, T. Kawata, Y. Saitoh, M. Sekine, T. Furuchi, I. Doi, S. Hirono, H. Homma
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Journal Title
J. Med. Chem.
Volume: 56, (5)
Issue: 5
Pages: 1894-1907
DOI
Related Report
Peer Reviewed
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[Journal Article] Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during the development in Caenorhabditis elegans2012
Author(s)
Yasuaki Saitoh, Masumi Katane, Tomonori Kawata, Kazuhiro Maeda, Masae Sekine, Takemitsu Furuchi, Hiroyuki Kobuna, Taro Sakamoto, Takao Inoue, Hiroyuki Arai, Yasuhito Nakagawa and Hiroshi Homma
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Journal Title
Mol. Cell. Biol
Volume: 32
Pages: 1967-1983
Related Report
Peer Reviewed
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[Journal Article] Spatiotemporal localization of D-amino acid oxidase and D-aspartate oxidases during development in Caenorhabditis elegans2012
Author(s)
Saitoh Y, Katane M, Kawata T, Maeda K, Sekine M, Furuchi T, Kobuna H, Sakamoto T, Inoue T, Arai H, Nakagawa Y, Homma H
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Journal Title
Mol Cell Biol
Volume: 32(10)
Issue: 10
Pages: 1967-1983
DOI
Related Report
Peer Reviewed
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[Presentation] D-Aspartate oxidase is involved in the caloric restriction-induced lifespan extension in C. elegans
Author(s)
Yasuaki Saitoh, Mari Okutsu, Masumi Katane, Masae Sekine, Takemitsu Furuchi, Taro Sakamoto, Takao Inoue, Hiroyuki Arai, Hiroshi Homma
Organizer
19th International C. elegans Meeting
Place of Presentation
University of California Los Angeles, California, USA
Related Report
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