| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Oxidized low-density lipoprotein (oxLDL) has been known as one of key factors for development of atherosclerosis. However, molecular features of in vivo oxLDL remain to be clarified. To characterize in vivo oxLDL, we performed an effective isolation of oxLDL from plasma followed by LC-ESI-MS/MS analysis of its lipid components. Combination of anion-exchange column separation of electronegative LDL (LDL(-)) and affinity purification using a monoclonal antibody against oxidized phosphatidylcholine (oxPC) revealed that oxLDL was enriched in LDL(-) fraction. In vivo oxLDL showed a different profile of PC molecular species from Cu-induced (in vitro) oxLDL. These data suggests that oxidized lipids in LDL may converted to other molecular species in vivo.
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