Identification and functional analysis of the transcriptonal complex regulating haematopoiesis

Research Project

Project/Area Number	24790090
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biological pharmacy
Research Institution	Teikyo University
Principal Investigator	MURATA Takuya 帝京大学, 薬学部, 助教 (00610106)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	血球分化 / 転写制御 / 遺伝子発現 / 転写共役因子 / 遺伝子発現制御 / 転写 / クロマチン
Research Abstract	To clarify molecular mechanism of C/EBPa transcriptional regulation in granulopoiesis, we tried to identify transcriptional cofactors of C/EBPa. As a result, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as an interactant of C/EBPa. PARP-1 co-represses C/EBPa transcriptional activity and reduces gene expression of C/EBPa target gene, myeloperoxidase (MPO). Additionally, PARP-1 binds C/EBPa response elements region and colocalizes with C/EBPa there. It is supposed that PARP-1 inhibits C/EBPa binding to response element.