| Project/Area Number |
24790095
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Meiji Pharmaceutical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | プロテオーム / リボソーム生合成 / NVL2 / DOB/MTR4 / SPF30 / WDR74 / プロテオミクス / 分子シャペロン |
|---|
| Research Abstract |
AAA family proteins regulate the dissociation of molecular complexes by their ATPase activities. Nucleolar AAA-ATPase NVL2 regulates the biogenesis of 60S ribosome via the interaction with RNA helicase DOB1/MTR4. Therefore, it is suggested that there are dissociating factors regulated by NVL2 in DOB1-containing complex. However, the dissociating proteins are not yet to be identified. In this study, the experimental system that DOB1-interacting proteins could be comprehensively identified was first established by proteomics using fluorescent two-dimensional difference gel electrophoresis and mass spectrometric analysis. Using this system, SPF30 and WDR74 were identified as the dissociating-factors regulated by NVL2 from DOB1-containing complex.
|
