• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms of anti-viral and anti-cancer activity of novel high mannose- binding lectins

Research Project

Project/Area Number 24790101
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionYasuda Women's University

Principal Investigator

SATO Yuichiro  安田女子大学, 薬学部, 講師 (60416427)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsレクチン / 抗ウイルス活性 / 抗がん活性 / オートファジー / 高マンノース糖鎖 / インテグリン / EGFR / 抗インフルエンザウイルス活性 / 抗がん作用
Research Abstract

Bacterial lectin (PFL) was cloned, expressed in Escherichia coli and purified. Glycan array screening has revealed that PFL preferentially recognizes high mannose glycans with alpha1-3 Man that was highly exposed at the D2 position. PFL showed a potent anti-influenza virus activity by inhibiting the virus entry into cells via interaction with virus surface glycoprotein HA. Furthermore, PFL showed cytotoxicity against human gastric cancer MKN28 cells. Upon treatment with exogenous PFL, both integrin alpha2 and EGFR on the cell surface were internalized to the cytoplasm and accumulated to perinuclear region, together with the bound PFL. As the results of integrin/EGFR accumulation in cytoplasm, autophagic cell death was induced. This is a novel anti-cancer mechanism induced by the lectin.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Expression shifts of host genes in early stage of virus infection or lectin binding2014

    • Author(s)
      Kinjiro Morimoto, Yuichiro Sato
    • Journal Title

      Journal of Yasuda Women's University

      Pages: 42-42

    • Related Report
      2013 Final Research Report
  • [Journal Article] Expression shifts of host genes in early stage of virus infection or lectin binding.2014

    • Author(s)
      Kinjiro Morimoto, Yuichiro Sato
    • Journal Title

      Journal of Yasuda Women’s University

      Volume: 42

    • Related Report
      2013 Annual Research Report
  • [Journal Article] High Mannose-Binding Antiviral Lectin PFL from Pseudomonas fluorescens Pf0-1 Promotes Cell Death of Gastric Cancer Cell MKN28 via Interaction with 2-integrin.2012

    • Author(s)
      Yuichiro Sato
    • Journal Title

      PLOS ONE

      Volume: 7 Issue: 9 Pages: 45922-45922

    • DOI

      10.1371/journal.pone.0045922

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 細菌レクチン(PFL)により誘導されるアノイキス様がん細胞死とオートファジーの関係2013

    • Author(s)
      佐藤雄一郎,久保貴紀,浅田真未,石田唯,宇川早紀,後藤瞳実,森本金次郎,瀬山敏雄
    • Organizer
      第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松山
    • Year and Date
      2013-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌レクチン(PFL)により誘導されるアノイキス様がん細胞死とオートファジーの関係2013

    • Author(s)
      佐藤雄一郎,久保貴紀,浅田真未,石田唯,宇川早紀,後藤瞳実,森本金次郎,瀬山敏雄
    • Organizer
      第52回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会
    • Place of Presentation
      松山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌Pseudomonas fluorescens Pf0-1由来レクチン(PFL)の抗ウイルス、抗がん作用の分子機構2012

    • Author(s)
      佐藤雄一郎,森本金次郎,久保貴紀,栁原五吉,瀬山敏雄
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌Pseudomonas fluorescens Pf0-1由来レクチン(PFL)の抗ウイルス、抗がん作用の分子機構2012

    • Author(s)
      佐藤雄一郎
    • Organizer
      第85回 日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi