| Project/Area Number |
24790103
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SHIMOZONO Satoshi 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (40391982)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 核膜 / 蛍光タンパク質 / 拡散 / 核膜の透過性 |
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| Research Abstract |
I studied the phenomenon of the high nuclear envelope (NE) permeability observed in the mouse fibroblast, Swiss 3T3 cell line. In this research, I obtained the following results by live cell imaging techniques using fluorescent proteins as probes for the permeability. First, the high NE permeability was not persistent but transient. Second, the calcium ion concentration in the cytoplasm, which is reported to affect the NE permeability, was not the cause of the high NE observed in Swiss3T3 cells. Third, this phenomenon was not specific only to the Swiss3T3 cell line but could be general because the high NE permeability was also observed in the NIH3T3 cell line and the HT1080 cell line.
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