Development of enzyme-responsive anticancer agents and fluorescent probes targeting the biosynthesis of nucleotides
| Project/Area Number |
24790108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 核酸の生合成 / カルボニル基のリン酸化 / ヌクレオチド / 酵素応答性分子 / イノシン / 蛍光標識化 / 抗がん剤 / 蛍光プローブ / アデニロコハク酸合成酵素 / CTP合成酵素 |
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| Research Abstract |
In this study, we developed a novel method to synthesize chemically modified analogs of inosine 6-phosphates, which are observed in the biosynthesis of nucleotides. For this purpose, we applied a non-nucleophilic acidic activator, N-(cyanomethyl)dimethylammonium triflate (CMMT), that we developed recently, to the phosphitylation of carbonyl oxygen by the phosphoramidite method, which enabled us to synthesize inosine 6-phosphate diesters bearing various substituents on the phosphate. Furthermore, we evaluated the stability of the inosine 6-phosphate diesters to find that they were stable in neutral aqueous solutions. These molecules will be useful for biological and pharmaceutical studies of the biosynthesis of nucleotides.
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Report
(3 results)
Research Products
(38 results)
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[Journal Article] Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans.2013
Author(s)
Nomura, Y, Onda, Y, Ohno, S, Taniguchi, H, Ando, K, Oka, N, Nishikawa, K, Yokogawa, T
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Journal Title
Protein Expr. Purif.
Volume: 88
Issue: 1
Pages: 13-19
DOI
Related Report
Peer Reviewed
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