| Project/Area Number |
24790109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
TAMURA Satoru 東北大学, 理学(系)研究科(研究院), 講師 (30362619)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 抗アレルギー / IgEレセプター / マスト細胞 / chlorin e6 / RBL 2H3 / pheophorbide a / PCA反応 / アレルギー |
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| Research Abstract |
Allergy is caused by chemical mediators degranulated from mast cells. This degranulation is derived through the important two steps. In a first step, immunoglobulin Es (IgEs) are attached on the IgE receptors on the surface of mast cells. Then, the IgEs on mast cells react with invaded allergens in a second step. In this circumstance, I wondered that the decrease of IgE receptors on the mast cells may be able to suppress allergic reactions. Based on this idea, I established the assay system for evaluation of the amount of IgE receptors on the surface of cultured mast cells by immunofluorescence staining and flow cytometry. By the screening from medicinal plants for inhibitors against expression of IgE receptors, chlorin e6 was found as a potent inhibitor. Fortunately, chlorin e6 suppressed the expression of IgE receptors not only in vitro but also in vivo rat experiments. Finally, chlorin e6 was clarified to inhibit in vivo allergic reaction of rats through po administration.
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