| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
Several artificial nucleic acids bearing 7-deazapurine-analog were synthesized and those torsion angles about glycosidic bond were estimated. We found that bridged nucleic acids have a similar glycosidic bond angle to RNA to stabilize the double stranded oligonucleotides. Moreover, nucleic acids bearing tetrahydropyran or cyclohehene as a sugar moiety were synthesized. These artificial nucleic acids showed a high resistance to 5'-3' exonuclease, and one of nucleic acids bearing a tetrahydropyran sugar present a high stabilization property of duplex oligonucleotides.
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