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Mercurial-resistance determinants in Pseudomonas strain K-62 plasmid pMR68

Research Project

Project/Area Number 24790128
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental pharmacy
Research InstitutionKitasato University

Principal Investigator

SONE YUKA  北里大学, 薬学部, 助教 (60550035)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsPseudomonas K-62 / 水銀耐性遺伝子 / pMR68 / 水銀浄化
Research Abstract

We report the complete nucleotide sequence of plasmid pMR68, isolated from Pseudomonas strain K-62, two plasmids contribute to broad-spectrum mercury resistance and that the mer operon from one of them (pMR26) has been previously characterized. The plasmid (pMR68) was 71,020 bp in length. Three mer gene clusters were identified. The first comprised merR-orf4-orf5-merT1-merP1-merF-merA-merB1, which confers bacterial resistance to mercuric ions and organomercury. The second and third clusters comprised merT2-merP2, which encodes a mercury transport system, and merB2, which encodes an organomercurial lyase, respectively. Escherichia coli cells carrying pMKY12 (containing merR-orf4-orf5-merT1-merP1-merF-merA-merB1 cloned from pMR68) were more resistant to, and volatilized more, mercury from mercuric ions and phenylmercury than the control cells. The novel mer gene identified in pMR68 may help us to design new strategies aimed at the bioremediation of mercurials.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Mercurial-resistance determinants in Pseudomonas sp. K-62 plasmid pMR68.2013

    • Author(s)
      Sone Y, Mochizuki Y, Koizawa K, Nakamura R, Pan-Hou H, Itoh T, Kiyono M
    • Journal Title

      AMB express

      Volume: 3(1) Issue: 1 Pages: 41-41

    • DOI

      10.1186/2191-0855-3-41

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 水銀耐性菌のメチル水銀トランスポーターに関する研究2013

    • Author(s)
      曽根有香、清野正子
    • Organizer
      平成25年度メチル水銀研究ミーティング
    • Place of Presentation
      東京
    • Year and Date
      2013-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 水銀トランスポーターMerC, MerE, MerF, MerTの機能解析2013

    • Author(s)
      曽根有香、中村亮介、芳生秀光、清野正子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Pseudomonas K-62由来plasmid pMR68の水銀耐性遺伝子の機能解析2012

    • Author(s)
      清野正子、曽根有香、中村亮介、芳生秀光
    • Organizer
      フォーラム2012 : 衛生薬学・環境トキシコロジー
    • Place of Presentation
      名古屋
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Pseudomonas K-62由来plasmid pMR68の水銀耐性遺伝子の機能解析

    • Author(s)
      清野 正子, 曽根 有香, 中村 亮介, 芳生 秀光
    • Organizer
      フォーラム2012:衛生薬学・環境トキシコロジー
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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