Antimicrobial effect of allograft and development of its preservation

Research Project

Project/Area Number	24790129
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Environmental pharmacy
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	NAKAMINAMI Hidemasa 東京薬科大学, 薬学部, 講師 (20548515)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000) Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords	Ａｌｌｏｇｒａｆｔ / 感染性心内膜炎 / 抗菌薬カクテル / 移植組織保存液 / MBC / FBC index / tryptophan代謝物質 / MIC / Chequerboard titrations法 / FIC index / 移植・再生医療 / 感染症 / 細菌 / 微生物 / 抗生物質
Outline of Final Research Achievements	In this study, I demonstrated that the mechanism of antimicrobial activity in allograft, which has been used in surgical therapy for the patients with infectious endocarditis, is the tryptophan metabolites. Additionally, I demonstrated that the cryopreserved allograft has the same or more antimicrobial activity than the fresh allograft. Furthermore, development of the brand new antimicrobial cocktail, which was able to prevent the bacterial contamination of allograft more effective than traditional one, was carried out in vitro experiment successfully.