Epigenome information profiling for molecular basis on seed epigetics
Project/Area Number |
24790137
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Environmental pharmacy
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Research Institution | National Institute of Health Sciences |
Principal Investigator |
NAKAMURA Kosuke 国立医薬品食品衛生研究所, 代謝生化学部, 主任研究官 (60570926)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 種子 / 遺伝子導入 / ゲノム / エピジェネティクス |
Research Abstract |
Genomic DNA and histone modifications, such as methylation, acetylation and phosphorylation, code for epigenetic information which control cellular function such as gene expression and regulation. This information is independent of the nucleic acid sequence, and is transmitted and maintained through cell division and generations. However, the epigenetic dynamisms among plants, especially dry seed to its germinal stage at individual grain level, have not been well characterized. In this study, an experimental model system was developed to analyze pattern of the genomic DNA methylation and histone modifications in genetically modified and wild-type rice. The obtained data were analyzed using multivariable statistical method. For the first time, the epigenetic information profiling showed that the genetically modified seed may differ from the wild-type seed.
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Report
(3 results)
Research Products
(82 results)
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[Journal Article] Development of pBT63, a positive control plasmid for qualitative detection of genetically modified rice2014
Author(s)
Minegishi, Y., Mano, J., Takabatake, R., Nakamura, K., Kondo, K., Kato, Y., Kitta, K., Akiyama, H
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Journal Title
Japanese Journal of Food Chemistry and Safety
Volume: 21
Pages: 48-56
NAID
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[Journal Article] Development of direct real-time PCR system applicable to a wide range of food and agricultural products2014
Author(s)
Mano, J., Hatano, S., Futo, S., Minegishi, Y., Ninomiya, K., Nakamura, K., Kondo, K., Teshima, R., Takabatake, R., Kitta, K
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Journal Title
Food Hygiene and Safety Science
Volume: 55
Pages: 25-33
NAID
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Peer Reviewed
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[Journal Article] Identification and detection method for genetically modified papaya resistant to papaya ringspot virus strains in Thailand2014
Author(s)
Nakamura, K., Kondo, K., Kobayashi, T., Noguchi, A., Ohmori, K., Takabatake, R., Kitta, K., Akiyama, H., Teshima, R., Nishimaki-Mogami, T
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Journal Title
Biological & Pharmaceutical Bulletin
Volume: 37
Pages: 1-5
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Peer Reviewed
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[Journal Article] Development of PCR primers designed for sensitive detection of genetically modified potato DNA in processed foods2013
Author(s)
Nakamura, K., Minamitake, Y., Nakamura, K., Kobayashi, T., Noguchi, A., Takabatake, R., Kitta, K., Hashimoto, H., Kawakami, H., Kondo, K., Teshima, R., Akiyama, H
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Journal Title
Japanese Journal of Food Chemistry and Safety
Volume: 20
Pages: 161-169
NAID
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Peer Reviewed
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[Journal Article] Evaluation of real-time PCR detection methods for detecting rice products contaminated by rice genetically modified with a CpTI—KDEL—T-nos transgenic construct2013
Author(s)
Nakamura, K., Akiyama, H., Kawano, N., Kobayashi, T., Yoshimatsu, K., Mano, J., Kitta, K., Ohmori, K., Noguchi, A., Kondo, K., Teshima, R
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Journal Title
Food Chemistry
Volume: 141
Pages: 2619-2624
Related Report
Peer Reviewed
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[Journal Article] Application of a qualitative and quantitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products2013
Author(s)
Nakamura, K., Akiyama, H., Takahashi, Y., Kobayashi, T., Noguchi, A., Ohmori, K., Kasahara, M., Kitta, K., Nakazawa, H., Kondo, K., Teshima, R
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Journal Title
Food Chemistry
Volume: 136
Pages: 895-901
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Peer Reviewed
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[Journal Article] Comparison of DNA extraction methods for sweet corn and processed sweet corns2013
Author(s)
Takabatake, R., Noritake, H., Noguchi, A., Nakamura, K., Kondo, K., Akiyama, H., Teshima, R., Mano, J., Kitta, K
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Journal Title
Food Hygiene and Safety Science
Volume: 54
Pages: 309-315
NAID
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Peer Reviewed
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[Journal Article] Interlaboratory validation study of an event-specific real-time polymerase chain reaction detection method for genetically modified 55-1 papaya2013
Author(s)
Noguchi, A., Nakamura, K., Sakata, K., Kobayashi, T., Akiyama, H., Kondo, K., Ohmori, K., Kasahara, M., Takabatake, R., Kitta, K., Teshima, R
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Journal Title
Journal of AOAC International
Volume: 96
Pages: 1054-1058
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Peer Reviewed
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[Journal Article] A novel DNA extraction and purification method using an ion-exchange resin type kit for the detection of genetically modified papaya in processed papaya products2013
Author(s)
Ohmori, K., Nakamura, K., Kasahara, M., Takabatake, R., Kitta, K., Fujimaki, T., Kondo, K., Teshima, R., Akiyama, H
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Journal Title
Food Control
Volume: 32
Pages: 728-735
Related Report
Peer Reviewed
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[Journal Article] Evaluation of real-time PCR detection methods for detecting rice products contaminated by rice genetically modified with a CpTI―KDEL―T-nos transgenic construct2013
Author(s)
Nakamura, K., Akiyama, H., Kawano, N., Kobayashi, T., Yoshimatsu, K., Mano, J., Kitta, K., Ohmori, K., Noguchi, A., Kondo, K., Teshima, R.
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Journal Title
Food Chemistry
Volume: 141
Pages: 2618-2624
Related Report
Peer Reviewed
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[Journal Article] Immobilized glycosylated Fmoc-amino acid for SPR: comparative studies of lectin-binding to linear or biantennary diLacNAc structures2013
Author(s)
Nakamura, K., Sakagami, H., Asanuma-Date, K., Nagasawa, N., Nakahara, Y., Akiyama, H., Ogawa, H.
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Journal Title
Carbohydrate Research
Volume: 382
Pages: 77-85
Related Report
Peer Reviewed
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[Journal Article] Development of PCR primers designed for sensitive detection of genetically modified potato DNA in processed foods2013
Author(s)
Nakamura, K., Minamitake, Y., Nakamura, K., Kobayashi, T., Noguchi, A., Takabatake, R., Kitta, K., Hashimoto, H., Kawakami, H., Kondo, K., Teshima, R., Akiyama, H.
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Journal Title
Japanese Journal of Food Chemistry and Safety,
Volume: 20
Pages: 161-169
NAID
Related Report
Peer Reviewed
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[Journal Article] Interlaboratory validation study of an event-specific real-time polymerase chain reaction detection method for genetically modified 55-1 papaya2013
Author(s)
Noguchi, A., Nakamura, K., Sakata, K., Kobayashi, T., Akiyama, H., Kondo, K., Ohmori, K., Kasahara, M., Takabatake, R., Kitta, K., Teshima, R.
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Journal Title
Journal of AOAC International
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Functional expression of amyloidogenic human stefins A and B in Pichia pastoris using codon optimization2013
Author(s)
Nakamura, K., Maeda,Y., Morimoto, K., Katayama, S., Kondo, K., Nakamura, S.
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Journal Title
Biotechnology and Applied Biochemistry
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Proficiency testing of unauthorized genetically modified rice using plasmid DNA test samples2012
Author(s)
Kasama, K., Inoue, Y, Akiyama, H., Suzuki, T., Sakata, K., Nakamura, K., Ohshima, Y., Kojima, K., Kondo, K., Teshima, R
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Journal Title
Japanese Journal of Food Chemistry and Safety
Volume: 19
Pages: 215-222
NAID
Related Report
Peer Reviewed
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[Journal Article] Quantification and identification of genetically modified maize events in non-identity preserved maize samples in 2009 using an individual kernel detection system2012
Author(s)
Akiyama, H., Minegishi, Y., Makiyama, D., Mano, J., Sakata, K., Nakamura, K., Noguchi, A., Takabatake, R., Futo, S., Kondo, K., Kitta, K., Kato, Y., Teshima, R.
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Journal Title
Food Hygiene and Safety Science
Volume: 53
Pages: 157-165
NAID
Related Report
Peer Reviewed
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[Journal Article] Comprehensive GMO detection using real-time PCR array : single-laboratory validation2012
Author(s)
Mano, J., Harada, M., Takabatake, R., Furui, S., Kitta, K., Nakamura K., Akiyama, H., Teshima, R., Noritake, H., Hatano, S., Futo, S., Minegishi, Y., Iizuka T
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Journal Title
Journal of AOAC International
Volume: 95
Pages: 508-516
Related Report
Peer Reviewed
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[Journal Article] Single-laboratory validation of comprehensive GMO detection method using real-time PCR array2012
Author(s)
Mano J., Harada, M., Hatano, S., Futo, S., Minegishi, Y., Noritake, H., Iizuka, T., Nakamura, K., Akiyama, H., Teshima, R., Takabatake, R., Furui, S., Kitta, K.
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Journal Title
Journal of AOAC International
Volume: 95
Pages: 508-516
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Peer Reviewed
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[Journal Article] Novel anti-HIV-1 activity produced by conjugating unsulfated dextran with polyL-lysine2012
Author(s)
Nakamura, K., Ohtsuki, T., Mori, H., Hoshino, H., Hoque, A., Oue, A., Kanou, F., Sakagami, H., Tanamoto, K., Ushijima, H., Kawasaki, N., Akiyama, H., Ogawa, H.
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Journal Title
Antiviral Research
Volume: 94
Pages: 89-97
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[Presentation] Immobilization method of glycosylated Fmoc-amino acid for SPR and interaction analysis between Pleurocybella porrigens lectin and carbohydrates2014
Author(s)
Fukasawa, A., Sakagami, H., Nakahara, Y., Nakamura, K., Ogawa, H.
Organizer
The 27th International Carbohydrate Symposium
Place of Presentation
Bangalore, India
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[Presentation] Novel monitoring scheme for authorized GM maize2013
Author(s)
Kitta, K., Kondo, K., Teshima, R., Nakamura, K., Noguchi, A., Takabatake, R., Mano, J
Organizer
GMCC-13
Place of Presentation
Portugal
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[Presentation] Characterization of the anti-HIV-1 mechanism of a pseudoproteoglycan produced by conjugating unsulfated dextran with poly-L-lysine2013
Author(s)
Ogawa, H., Kano, F., Otsuki, T., Hoshino, H., Nakamura, K., Mori, H., Sakagami, H.
Organizer
The 22nd International Symposium on Glycoconjugates
Place of Presentation
Dalian, China
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[Presentation] Novel monitoring scheme for authorized GM maize2013
Author(s)
Kitta, K., Kondo, K., Teshima, R., Nakamura, K., Noguchi, A., Takabatake, R., Mano, J.
Organizer
GMCC-13
Place of Presentation
Lisbon, Portugal
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[Presentation] ツキヨタケおよび近縁種のPCR-RFLPを用いた迅速同定法の検討2013
Author(s)
菅野陽平, 坂田こずえ, 野口秋雄,中村公亮, 小林友子, 福田のぞみ, 佐藤正幸, 最上(西巻)知子, 手島玲子, 長澤栄史,近藤一成
Organizer
第106回 日本食品衛生学会学術講演会
Place of Presentation
沖縄
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[Presentation] 2010年度米国産不分別遺伝子組換えトウモロコシ試料中の系統分析2013
Author(s)
野口秋雄, 坂田こずえ, 真野潤一, 中村公亮, 高畠令王奈, 峯岸恭孝, 橘田和美,穐山浩, 手島玲子, 近藤一成, 最上(西巻)知子
Organizer
第106回 日本食品衛生学会学術講演会
Place of Presentation
沖縄
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[Presentation] Evaluation of real-time PCR detection methods for detecting genetically modified Bt rice lines harboring CpTI—KDEL—T-nos transgenic construct in rice product2012
Author(s)
Nakamura, K., Akiyama, H., Kawano, N., Kobayashi, T., Yoshimatsu, K., Mano, J., Kitta, K., Noguchi, A., Kondo, K., Teshima, R
Organizer
2012 ISNFF-Functional Foods
Place of Presentation
Hawaii, USA
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[Presentation] Applicability of Qualitative and Quantitative Real-time Polymerase Chain Reaction Method for Detecting Genetically Modified Papaya Line 55-1 to Papaya Products2012
Author(s)
Nakamura, K., Akiyama, H., Kobayashi, T., Noguchi, A., Ohmori, K., Kasahara, M., Kitta, K., Kondo, K., Teshima, R
Organizer
126th AOAC Annual Meeting & Exposition, Las Vegas
Place of Presentation
USA
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[Presentation] Applicability of qualitative and quantitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 to papaya products2012
Author(s)
Nakamura, K., Akiyama, H., Kobayashi, T., Noguchi, A., Ohmori, K., Kasahara, M., Kitta, K., Kondo, K., Teshima, R.
Organizer
126th AOAC Annual Meeting & Exposition
Place of Presentation
Las Vegas, USA
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[Presentation] Amyloidogenicities of artificially synthesized human stefins A and B2012
Author(s)
Morimoto, K., Katayama, S., Fukumoto, T., Nakamura, K., Nakamura, S.
Organizer
2012 ISNFF-Functional Foods, Nutraceuticals, Natural Health Products and Dietary Supplements
Place of Presentation
Hawaii, USA
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[Presentation] Evaluation of real-time PCR detection methods for detecting genetically modified Bt rice lines harboring CpTI-KDEL-T-nos transgenic construct in rice product2012
Author(s)
Nakamura, K., Akiyama, H., Kawano, N., Kobayashi, T., Yoshimatsu, K., Mano, J., Kitta, K., Noguchi, A., Kondo, K., Teshima, R.
Organizer
2012 ISNFF-Functional Foods, Nutraceuticals, Natural Health Products and Dietary Supplements
Place of Presentation
Hawaii, USA
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[Presentation] Apple procyanidins inhibit development of collagen-induced arthritis via down-regulation of Th17 response2012
Author(s)
Nakamura, K., Matsuoka, H., Nakashima, S., Kanda, T., Kondo, K., Teshima, R., Akiyama, H.
Organizer
2012 ISNFF-Functional Foods, Nutraceuticals, Natural Health Products and Dietary Supplements
Place of Presentation
Hawaii, USA
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