Development of novel claudin-4 binder and its application in oral vaccine
| Project/Area Number |
24790155
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
WATARI Akihiro 大阪大学, 薬学研究科(研究院), 助教 (80452465)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ワクチン / claudin / DDS / 免疫学 / クローディン |
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| Research Abstract |
We prepared C-CPE mutant, which had higher affinity for claudin-4 than C-CPE, and made a fusion protein with the C-CPE mutant and ovalbumin (OVA) as a model antigen. Immunization with the C-CPE mutant-fused OVA led to increased anti-tumor activity relative to that of mice immunized with the C-CPE-fused OVA. The liposomes modified with the C-CPE mutant bind to CL-4-expressing cells and targeted to Peyer's patch cells in mouse intestine. These findings suggest that the modified liposome shows promise as a claudin-targeted oral vaccine.
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Report
(3 results)
Research Products
(48 results)
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[Presentation] Evaluation of HCV infection and replication using human iPS cell-derived hepatocytes2012
Author(s)
Yagi K., Yoshida T., Yamane S., Takayama K., Watari A., Kondoh M., Sakurai F., Sakamoto N., Mtsuura Y., Mizuguchi H
Organizer
HCV2012 19th International Symposium on Hepatittis C virus and Relate viruses
Place of Presentation
Venice, ITALY
Related Report
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[Presentation] Biochemical analysis of a claudin ligand that exhibits a broad binding specificity2012
Author(s)
Doyama R., Suzuki H., Matsushita K., Li X, Takahashi A., Matsuhisa K., Tsujino H., Watari A., Kondoh M., Aoyama H., Uno T. and Yagi K
Organizer
Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
Place of Presentation
Merida, MEXICO
Related Report
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[Presentation] Biochemical analysis of a claudin ligand that exhibits a broad binding specificity2012
Author(s)
Doyama R. ,Suzuki H., Matsushita K., Li X., Takahashi A., Matsuhisa K., Tsujino H., Watari A., Kondoh M. Aoyama H., Uno T. and Yagi K.
Organizer
Molecular Structure and Funcction of the Apical Junctional Comples in Epithelia and Endothelia
Place of Presentation
Merida,MEXICO
Related Report
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