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Development of rapid and simple method for determination of monoclonal antibody drug and its application to clinical samples

Research Project

Project/Area Number 24790158
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionNagasaki University

Principal Investigator

OHYAMA KANAME  長崎大学, 医歯(薬)学総合研究科, 准教授 (50437860)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords臨床化学 / 抗体医薬 / 質量分析 / 血清 / 抗体医薬品
Research Abstract

We developed capillary electrophoresis-tandem mass spectrometry for determination of monoclonal antibody drug in human plasma. In order to selectively determine the Fab peptides derived from the drug after digestion, measurement parameters were optimized. Three peptides were selected and were determined if these were useful to monitor as a surrogate peptide. As a result, the MS signal from one peptide was found to increase as the concentration of antibody drug increased. The combination of this method with the magnetic beads immobilized with TNF-alpha will improved the selectivity for the determination of the antibody drug.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Optimization of separation and digestion conditions in immune complexome analysis2013

    • Author(s)
      M. Baba, K. Ohyama, N. Kishikawa, N. Kuroda
    • Journal Title

      Analytical Biochemistry

      Volume: 443 Pages: 181-186

    • NAID

      120006986089

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum immune complex containing thrombospondin-1 : a novel biomarker for early rheumatoid arthritis2012

    • Author(s)
      K. Ohyama, A. Kawakami, M. Tamai, M. Baba, N. Kishikawa, N. Kuroda
    • Journal Title

      Annals of the Rheumatic Diseases

      Volume: 71 Pages: 1916-1917

    • NAID

      120006985489

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immune complexome analysis of serum and its application in screening for immune complex antigens in rheumatoid arthritis.2011

    • Author(s)
      Ohyama K, Ueki Y, Kawakami A, Kishikawa N, Tamai M, Osaki M, Kamihira S, Nakashima K, Kuroda N.
    • Journal Title

      Clin Chem

      Volume: 57 (6) Issue: 6 Pages: 905-909

    • DOI

      10.1373/clinchem.2010.157776

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] イムノコンプレキソーム解析における免疫複合体の捕集方法についての検討-Protein G、Protein A固定化磁気ビーズの比較-2013

    • Author(s)
      大山要,吉見春香、馬場雅子、岸川直哉、黒田直敬
    • Organizer
      第7回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] イムノコンプレキソーム解析による自己抗原プロファイリングに基づく自己免疫疾患へのアプローチ2013

    • Author(s)
      馬場雅子,大山要、一瀬邦弘、玉井慎美、岸川直哉、川上純、黒田直敬
    • Organizer
      第7回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] イムノコンプレキソーム解析における免疫複合体の捕集方法についての検討-Protein G、Protein A固定化磁気ビーズの比較-2013

    • Author(s)
      大山 要、吉見春香、馬場雅子、岸川直哉、黒田直敬
    • Organizer
      第7回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      仙台市、東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] イムノコンプレキソーム解析による自己抗原プロフィリングに基づく自己免疫疾患へのアプローチ2013

    • Author(s)
      馬場雅子、大山 要、一瀬邦弘、玉井慎美、岸川直哉、川上 純、黒田直敬
    • Organizer
      第7回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      仙台市、東北大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] イムノコンプレキソーム解析による自己免疫疾患患者の抗原プロファイリング2013

    • Author(s)
      大山 要、馬場雅子、玉井慎美 他
    • Organizer
      第24回日本臨床化学会九州支部総会
    • Place of Presentation
      沖縄
    • Related Report
      2012 Research-status Report
  • [Presentation] イムノコンプレキソーム解析法におけるペプチド分離およびトリプシン消化の最適化2012

    • Author(s)
      馬場雅子,大山要,岸川直哉,黒田直敬
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 免疫疾患に関連する免疫複合体抗原のスクリーニングへのイムノコンプレキソーム解析法の応用2012

    • Author(s)
      大山要,川上純,玉井慎美,馬場雅子,岸川直哉,黒田直敬
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 早期関節リウマチ患者のイムノコンプレキソーム解析2012

    • Author(s)
      大山要,馬場雅子,川上純,玉井慎美,岸川直哉,植木幸孝,上平憲,中島憲一郎,黒田直敬
    • Organizer
      第23回日本臨床化学会九州支部総会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Book] Immune complexome analysis. Advances in Clinical Chemistry volume 60, Ed. by G. Makowski, Elsevier, Chapter 42013

    • Author(s)
      K. Ohyama, N. Kuroda
    • Related Report
      2013 Final Research Report
  • [Book] Advances in Clinical Chemistry volume 602013

    • Author(s)
      Kaname Ohyama, Naotaka Kuroda
    • Total Pages
      13
    • Publisher
      Elsevier
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/analysis/index-j.html

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 中枢神経ループス(NPSLE)診断用バイオマーカー2013

    • Inventor(s)
      一瀬邦弘,大山 要,川上 純,黒 田直敬,中嶋秀樹,岸川直哉,馬場雅子
    • Industrial Property Rights Holder
      国立大学法人長崎大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-055543
    • Filing Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 中枢神経ループス(NPSLE)診断用バイオマーカー2013

    • Inventor(s)
      一瀬邦弘、大山要、川上純、黒田直敬、中嶋秀樹、岸川直哉、馬場雅子
    • Industrial Property Rights Holder
      国立大学法人 長崎大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-055543
    • Filing Date
      2013-03-18
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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