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Embryological analysis of mouse Multilineage-differentiating stress enduring (Muse) cells.

Research Project

Project/Area Number 24790188
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTohoku University

Principal Investigator

WAKAO Shohei  東北大学, 医学(系)研究科(研究院), 助教 (80511948)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords多能性幹細胞
Outline of Final Research Achievements

Muse cells can be isolated from mesenchymal stem cells by FACS as cells positive for stage-specific embryonic antigen-3 (SSEA-3), a marker for undifferentiated human pluripotent stem cells. However, SSEA-3 is a glycolipid, it is difficult to embryological analysis. In order to search for the new marker, Muse cells were subjected to FACS analysis using various antibodies. From these results, it was suggested that Cripto-1 was a new marker in substitution for SSEA-3. Therefore, we constructed several lines of transgenic mice expressing GFP under the control of Cripto-1 promoter and subjected to histological analysis of these mice connective tissue. The GFP and SSEA-3 double positive cells were located sparsely in the connective tissue of the hypodermis and do not associated with particular structures such as blood vessels. These transgenic mice will be useful tool for future investigation of embryological analysis of Muse cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 2 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Mesenchymal stem cells as a source of Schwann cells: their anticipated used in perioheral nerve regeneration.2015

    • Author(s)
      Wakao S, Matsuse D, Dezawa M.
    • Journal Title

      Cells Tissues Organs

      Volume: 200 Issue: 1 Pages: 1-11

    • DOI

      10.1159/000368188

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human adipose tissue possesses a unique population of pluripotent stem cells with non-tumorigenic and low telomerase activities : potential implications in regenerative medicine2014

    • Author(s)
      Ogura F, Wakao S, Kuroda Y, Tsuchiyama K, Bagheri M, Heneidi S, Chazenbalk G, Aiba S, Dezawa M
    • Journal Title

      Stem Cells Dev

      Volume: 23(7) Issue: 7 Pages: 717-28

    • DOI

      10.1089/scd.2013.0473

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Muse cells, a novel type of non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues2014

    • Author(s)
      Wakao S, Akashi H, Kushida Y, Dezawa M.
    • Journal Title

      Pathol Int

      Volume: 64(1) Issue: 1 Pages: 1-9

    • DOI

      10.1111/pin.12129

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (muse) cells, distinct stem cells in human fibroblasts2013

    • Author(s)
      Tsuchiyama K, Wakao S, Kuroda Y, Ogura F, Nojima M, Sawaya N, Yamasaki K, Aiba S, Dezawa M
    • Journal Title

      J Invest Dermatol

      Volume: 133(10) Issue: 10 Pages: 2425-35

    • DOI

      10.1038/jid.2013.172

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel approach to collect satellite cells from adult skeletal muscles based on their stress tolerance2013

    • Author(s)
      Shigemoto T, Kuroda Y, Wakao S, Dezawa M
    • Journal Title

      STEM CELLS Translational Medicine

      Volume: 2(7) Issue: 7 Pages: 488-98

    • DOI

      10.5966/sctm.2012-0130

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation, culture and evaluation of Multilineage-differentiating Stress Enduring (Muse) cells2013

    • Author(s)
      Kuroda Y, Wakao S, Kitada M, Murakami T, Nojima M, and Dezawa M
    • Journal Title

      Nat Protoc

      Volume: 8(7) Issue: 7 Pages: 1391-415

    • DOI

      10.1038/nprot.2013.076

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques2013

    • Author(s)
      Hayashi T, Wakao S, Kitada M, Ose T, Watabe H, Kuroda Y, Mitsunaga K, Matsuse D, Shigemoto T, Ito A, Ikeda H, Fukuyama H, Onoe H, Tabata Y, Dezawa M
    • Journal Title

      J Clin Invest

      Volume: 123(1) Issue: 1 Pages: 272-284

    • DOI

      10.1172/jci62516

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The elite and stochastic model for iPS cell generation: multilineage-differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells2013

    • Author(s)
      Wakao S, Kitada M, Dezawa M.
    • Journal Title

      Cytometry A

      Volume: 83 Issue: 1 Pages: 18-26

    • DOI

      10.1002/cyto.a.22069

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.2012

    • Author(s)
      Wakao S, Kitada M, Kuroda Y, Ogura F, Murakami T, Niwa A, Dezawa M
    • Journal Title

      PLoS One

      Volume: e48677

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transplantation of bone marrow stromal cells-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection.2012

    • Author(s)
      Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera JJ, Kawashima R, Tominaga T, Dezawa M
    • Journal Title

      Cell Transplantation

      Volume: -

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒトMuse細胞内に存在するサブポピュレーション2015

    • Author(s)
      若尾昌平、黒田康勝、出澤真理
    • Organizer
      日本再生医療学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトMuse細胞内に存在するサブポピュレーションの特性2014

    • Author(s)
      若尾昌平、黒田康勝、出澤真理
    • Organizer
      第119回日本解剖学会総会全国学術集会
    • Place of Presentation
      自治医科大学キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト生体由来多能性幹細胞(Muse細胞)内に存在するサブポピュレーションの解析2014

    • Author(s)
      若尾昌平、黒田康勝、出澤真理
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 多様なコロニーからヒトiPS細胞を選別するための形態的特徴と遺伝子発現2013

    • Author(s)
      若尾昌平、北田容章、黒田康勝、小椋文貴、村上徹、出澤真理
    • Organizer
      日本解剖学会全国学術集会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 多様なコロニーからヒトiPS細胞を選別するための形態的特徴と遺伝子発現2012

    • Author(s)
      若尾昌平、北田容章、黒田康勝、小椋文貴、村上徹、出澤真理
    • Organizer
      日本解剖学会東北・北海道連合支部学術集会
    • Place of Presentation
      山形大学医学部大講義室
    • Related Report
      2012 Research-status Report
  • [Book] 膵島の再生医療2015

    • Author(s)
      若尾昌平、出澤真理
    • Total Pages
      173
    • Publisher
      診断と治療社
    • Related Report
      2014 Annual Research Report
  • [Book] 最新医学2014

    • Author(s)
      若尾昌平、串田良祐、出澤真理
    • Total Pages
      745
    • Publisher
      最新医学社
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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